Bacteriophages (phages), highly prevalent in aquatic and terrestrial environments, have emerged as novel antimicrobial agents in food and agricultural systems. Owing to their efficient and unique infection mechanism, phages offer an alternative to antibiotic therapy as they specifically target their host bacteria without causing antibiotic resistance. However, the real-world applications of phages as antimicrobials are still limited due to their low survivability under harsh conditions and reduced antimicrobial efficacy. There is an unmet need to understand the challenges of using phages in food and agricultural systems and potential strategies to enhance their stability and delivery. This review overviews the challenges of using phages, including acidic conditions, improper temperatures, UV-light irradiation, desiccation, and inefficient delivery. It also summarizes novel strategies such as encapsulation, embedding, and immobilization, which enable improved viability and enhanced delivery. The protein capsid and nucleic acid components of phages are delicate and sensitive to physicochemical stresses. Incorporating phages into biocompatible materials can provide a physical barrier for improving phage stability and enhancing phage delivery, resulting in a high antimicrobial efficacy. In conclusion, the development of phage delivery systems can significantly overcome the challenges associated with phage treatments and reduce the risk of foodborne diseases in the industry.
Keywords: Bacteriophages; antimicrobial efficacy; biocontrol; encapsulation; phage delivery systems; phage stability.