Comprehensive assessment of the estrogenic activity of resin composites

Hyeyeong Seo; Yu-Jin Ahn; Huiwon Seo; Ahreum Seo; Hayeon Lee; Seok-Hee Lee; Won-Jun Shon; Yooheon Park

doi:10.1016/j.chemosphere.2023.140104

Comprehensive assessment of the estrogenic activity of resin composites

Chemosphere. 2023 Dec:343:140104. doi: 10.1016/j.chemosphere.2023.140104. Epub 2023 Sep 9.

Authors

Hyeyeong Seo¹, Yu-Jin Ahn², Huiwon Seo¹, Ahreum Seo¹, Hayeon Lee¹, Seok-Hee Lee³, Won-Jun Shon², Yooheon Park⁴

Affiliations

¹ Department of Food Science and Biotechnology, Dongguk University, Goyang, 10326, Republic of Korea.
² Department of Conservative Dentistry, Dental Research Institute and School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea.
³ Department of Food Science and Biotechnology, Dongguk University, Goyang, 10326, Republic of Korea; Department of Food and Medical Products Regulatory Policy, Dongguk University, Seoul, 04620, Republic of Korea.
⁴ Department of Food Science and Biotechnology, Dongguk University, Goyang, 10326, Republic of Korea; Department of Food and Medical Products Regulatory Policy, Dongguk University, Seoul, 04620, Republic of Korea. Electronic address: ypark@dongguk.edu.

PMID: 37696476
DOI: 10.1016/j.chemosphere.2023.140104

Abstract

Resin-based dental composites have been developed to restore decayed teeth or modify tooth color due to their excellent physical and chemical properties. Such composites may have intrinsic toxicity due to components released into the mouth during the early stage of polymerization, and afterward as a result of erosion or material decomposition. In addition, resin-based dental composites have potential environmental pollutant by elution of monomers and degradation. Since certain monomers of resin matrices are synthesized from bisphenol A (BPA), which acts as an estrogenic endocrine disruptor, these resin matrices may have estrogenic activity. Therefore, the estrogenic endocrine-disrupting activity of various dental composites should be evaluated. In this study, we evaluated the estrogenic endocrine-disrupting activity of 10 resin composites by using a BRET-based estrogen receptor (ER)α and ERβ dimerization assays and ER transactivation assay. BPA, BisDMA, BisGMA, BisEMA, TEGDMA, HMBP, and DMPA mediated ERα dimerization, and BPA, BisDMA, and DMPA also mediated ERβ dimerization. Except for UDMA and CQ, all the compounds were identified as estrogen agonists or antagonists. In-depth information for the safe use of dental composites was acquired, and it was confirmed how the component of dental composites acts in the ER signaling pathway. Further studies on the low-dose and long-term release of these compounds are needed to ensure the safe use of these resin-based dental composites.

Keywords: Bioluminescence resonance energy transfer; Dimerization assay; Estrogen receptor; Resin composites; Transactivation assay.