High-sensitivity hemoglobin detection based on polarization-differential spectrophotometry

Chunlan Deng; Qilai Zhao; Yichuan Gan; Changsheng Yang; Hongbo Zhu; Shiman Mo; Junjie Zheng; Jialong Li; Kui Jiang; Zhouming Feng; Xiaoming Wei; Qinyuan Zhang; Zhongmin Yang; Shanhui Xu

doi:10.1016/j.bios.2023.115667

High-sensitivity hemoglobin detection based on polarization-differential spectrophotometry

Biosens Bioelectron. 2023 Dec 1:241:115667. doi: 10.1016/j.bios.2023.115667. Epub 2023 Sep 9.

Authors

Chunlan Deng¹, Qilai Zhao², Yichuan Gan³, Changsheng Yang⁴, Hongbo Zhu⁵, Shiman Mo¹, Junjie Zheng⁶, Jialong Li⁶, Kui Jiang¹, Zhouming Feng¹, Xiaoming Wei⁶, Qinyuan Zhang⁷, Zhongmin Yang⁸, Shanhui Xu⁹

Affiliations

¹ School of Materials of Science and Engineering, South China University of Technology, Guangzhou, 510640, China; State Key Laboratory of Luminescent Materials and Devices and Institute of Optical Communication Materials, South China University of Technology, Guangzhou, 510640, China.
² School of Physics and Optoelectronics, South China University of Technology, Guangzhou, 510640, China; State Key Laboratory of Luminescent Materials and Devices and Institute of Optical Communication Materials, South China University of Technology, Guangzhou, 510640, China. Electronic address: zhaoql@scut.edu.cn.
³ The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510630, China.
⁴ State Key Laboratory of Luminescent Materials and Devices and Institute of Optical Communication Materials, South China University of Technology, Guangzhou, 510640, China; Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices, Guangzhou, 510640, China; Hengqin Firay Sci-Tech Company Ltd., Zhuhai, 519031, China.
⁵ State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China.
⁶ School of Physics and Optoelectronics, South China University of Technology, Guangzhou, 510640, China; State Key Laboratory of Luminescent Materials and Devices and Institute of Optical Communication Materials, South China University of Technology, Guangzhou, 510640, China.
⁷ School of Physics and Optoelectronics, South China University of Technology, Guangzhou, 510640, China; State Key Laboratory of Luminescent Materials and Devices and Institute of Optical Communication Materials, South China University of Technology, Guangzhou, 510640, China; Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices, Guangzhou, 510640, China; Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, South China University of Technology, Guangzhou, 510640, China.
⁸ School of Physics and Optoelectronics, South China University of Technology, Guangzhou, 510640, China; State Key Laboratory of Luminescent Materials and Devices and Institute of Optical Communication Materials, South China University of Technology, Guangzhou, 510640, China; Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices, Guangzhou, 510640, China; Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, South China University of Technology, Guangzhou, 510640, China; Guangdong Engineering Technology Research and Development Center of High-performance Fiber Laser Techniques and Equipments, Zhuhai, 519031, China.
⁹ School of Materials of Science and Engineering, South China University of Technology, Guangzhou, 510640, China; School of Physics and Optoelectronics, South China University of Technology, Guangzhou, 510640, China; State Key Laboratory of Luminescent Materials and Devices and Institute of Optical Communication Materials, South China University of Technology, Guangzhou, 510640, China; Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices, Guangzhou, 510640, China; Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, South China University of Technology, Guangzhou, 510640, China; Guangdong Engineering Technology Research and Development Center of High-performance Fiber Laser Techniques and Equipments, Zhuhai, 519031, China; Hengqin Firay Sci-Tech Company Ltd., Zhuhai, 519031, China. Electronic address: flxshy@scut.edu.cn.

PMID: 37696221
DOI: 10.1016/j.bios.2023.115667

Abstract

Hemoglobin content is recognized as a momentous and fundamental physiological indicator, especially the precise detection of trace hemoglobin is of great significance for early diagnosis and prevention of tumors, cancer, organic injury, etc. Therefore, high-sensitivity hemoglobin detection is imperative. However, effective detection methods and reliable detection systems are still lacking and remain enormous challenges. Herein, we present a synthetical strategy to break through the existing bottleneck based on polarization-differential spectrophotometry and high-performance single-frequency green fiber laser. Importantly, this framework not only has precisely extracted the two-dimensional information of intensity and polarization during the interaction between laser and hemoglobin, but also has taken advantage of the high monochromaticity and fine directivity in the optimized laser source to reduce the undesirable scattered disturbance. Thus, the hemoglobin detection sensitivity of 7.2 × 10^-5 g/L has advanced a hundredfold compared with conventional spectrophotometry, and the responsive dynamic range is close to six orders of magnitude. Results indicate that our technology can realize high-sensitivity detection of trace hemoglobin content, holding promising applications for precision medicine and early diagnosis as an optical direct and fast detection method.

Keywords: Hemoglobin; High-sensitivity; Polarization difference; Single-frequency fiber laser; Spectrophotometry.

MeSH terms

Biosensing Techniques*
Hemoglobins / analysis
Lasers
Light
Spectrophotometry / methods

Substances

Hemoglobins