Recovery of 52 bacterial genomes from the fecal microbiome of the domestic cat (Felis catus) using Hi-C proximity ligation and shotgun metagenomics

Connie A Rojas; Jennifer Gardy; Jonathan A Eisen; Holly H Ganz

doi:10.1128/MRA.00601-23

Recovery of 52 bacterial genomes from the fecal microbiome of the domestic cat (Felis catus) using Hi-C proximity ligation and shotgun metagenomics

Microbiol Resour Announc. 2023 Oct 19;12(10):e0060123. doi: 10.1128/MRA.00601-23. Epub 2023 Sep 11.

Authors

Connie A Rojas¹, Jennifer Gardy², Jonathan A Eisen³, Holly H Ganz¹

Affiliations

¹ AnimalBiome, Oakland, California, USA.
² Bill & Melinda Gates Foundation, Seattle, Washington, USA.
³ Evolution and Ecology, University of California, Davis, California, USA.

Abstract

We used Hi-C proximity ligation with shotgun sequencing to retrieve metagenome-assembled genomes (MAGs) from the fecal microbiomes of two domestic cats (Felis catus). The genomes were assessed for completeness and contamination, classified taxonomically, and annotated for putative antimicrobial resistance (AMR) genes.

Keywords: Hi-C; antimicrobial-resistance; assembly; domestic cats; fecal microbiome; gut bacteria; gut microbiome; metagenome-assembled genomes (MAGs); shotgun metagenomics.

Abstract

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