Preparation of a Pt(II)-3-Hydroxy-2-tolyl-4 H-chromen-4-one Complex Having Antimicrobial, Anticancerous, and Radical Scavenging Activities with Related Computational Studies

Masrat Mohmad; Nivedita Agnihotri; Vikas Kumar; Mohammad Azam; Raj Kamal; Ashish Kumar; Ujjawal Sharma; Saleem Javed; Sambantham Muthu; Kim Min

doi:10.1021/acsomega.3c01316

Preparation of a Pt(II)-3-Hydroxy-2-tolyl-4 H-chromen-4-one Complex Having Antimicrobial, Anticancerous, and Radical Scavenging Activities with Related Computational Studies

ACS Omega. 2023 Aug 22;8(35):31648-31660. doi: 10.1021/acsomega.3c01316. eCollection 2023 Sep 5.

Authors

Masrat Mohmad¹, Nivedita Agnihotri¹, Vikas Kumar², Mohammad Azam³, Raj Kamal⁴, Ashish Kumar⁴, Ujjawal Sharma⁵, Saleem Javed⁶, Sambantham Muthu⁷, Kim Min⁸

Affiliations

¹ Department of Chemistry, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133207, India.
² Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133207, India.
³ Department of Chemistry, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
⁴ Department of Chemistry, Kurukshetra University, Kurukshetra 136119, India.
⁵ Department of Human Genetics and Molecular Medicine, School of Health Sciences, Central University of Punjab, Bhatinda 151401, India.
⁶ Department of Chemistry, Jamia Millia Islamia, New Delhi 110025, India.
⁷ Department of Physics, Aringnar Anna Govt. Arts College, Cheyyar 604407, India.
⁸ Department of Safety Engineering, Dongguk University, 123 Dongdae-ro, Gyeongju 780714, Gyeongbuk, South Korea.

Abstract

A novel benzopyran-based platinum (II)-3-hydroxy-2-tolyl-4H-chromen-4-one (HToC) complex has been prepared and studied by UV-visible spectrophotometry. The study is based on the colored complexation between Pt(II) and HToC in the pH range of 8.92-9.21, resulting in the formation of a stable binary yellow complex exhibiting λ_max at 509-525 nm. The formed complex maintains linearity between 0.0 and 1.8 μg Pt(II) mL^-1. The well-known qualitative analytical methods, including Job's method of continuous variations and the mole ratio approach, have both proven that the stoichiometry of the complex is 1:2 [Pt(II)/HToC]. Hence, the analytical results suggest that the formed platinum complex exhibits a square planar geometry. The values of various attributes corresponding to spectrophotometric studies and statistical calculations, such as the molar extinction coefficient (6.790 × 10⁴ L mol^-1 cm^-1), Sandell's sensitivity (0.0029 μg Pt(II) cm^-2), standard deviation (± 0.0011), RSD (0.317%), limit of detection (0.0147 μg mL^-1) and correlation coefficient (0.9999), show that the performed study satisfies all of the criteria for good sensitivity, versatility, and cost-effectiveness. In order to have an apprehension of the molecular geometry and other structural specifics of the complex, DFT studies have been carried out. The in vitro anticancer potential of the ligand and its platinum complex in the human breast cancer cell line (T-27D), as determined by the MTT assay, reveals that the complex has better antiproliferative potential than the ligand. The antimicrobial potential of the complex has been successfully tested against both Gram-positive and -negative bacteria. Antioxidant capacity results suggest the better radical scavenging capacity of the complex than that of the ligand.