Long non-coding RNAs influence aging process of sciatic nerves in SD rats

Rui Kuang; Yi Zhang; Guanggeng Wu; Zhaowei Zhu; Shuqia Xu; Xiangxia Liu; Yangbin Xu; Yunxiang Luo

doi:10.2174/1386207326666230907115800

Long non-coding RNAs influence aging process of sciatic nerves in SD rats

Comb Chem High Throughput Screen. 2023 Sep 7. doi: 10.2174/1386207326666230907115800. Online ahead of print.

Authors

Rui Kuang¹, Yi Zhang¹, Guanggeng Wu¹, Zhaowei Zhu¹, Shuqia Xu¹, Xiangxia Liu², Yangbin Xu¹, Yunxiang Luo¹

Affiliations

¹ Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, China
² Department of Plastic Surgery, University of Tennessee Health Science Center, Memphis, TN, United States

PMID: 37691192
DOI: 10.2174/1386207326666230907115800

Abstract

Objectives: To investigate the long non-coding RNAs (lncRNAs) changes in the sciatic nerve (SN) in Sprague Dawley (SD) rats during aging.

Methods: Eighteen healthy SD rats were selected at the age of 1 month (1M) and 24 months (24M) and SNs were collected. High-throughput transcriptome sequencing and bioinformatics analysis were performed. Protein-protein interaction (PPI) networks and competing endogenous RNA (ceRNA) networks were established according to differentially expressed genes (DEGs).

Result: As the length of lncRNAs increased, its proportion to the total number of lncRNAs decreased. A total of 4079 DElncRNAs were identified in Con vs. 24M. GO analysis was primarily clustered in nerve and lipid metabolism, extracellular matrix, and vascularization-related fields. There were 17 nodes in the PPI network of the target genes of up-regulating genes including Itgb2, Lox, Col11a1, Wnt5a, Kras, etc. Using quantitative RT-PCR, microarray sequencing accuracy was validated. There were 169 nodes constructing the PPI network of down-regulated target genes, mainly including Col1a1, Hmgcs1, Hmgcr. CeRNA interaction networks were constructed

Conclusion: Lipid metabolism, angiogenesis, and ECM fields might play an important role in the senescence process in SNs. Col3a1, Serpinh1, Hmgcr, and Fdps could be candidates for nerve aging research.

Keywords: Long non-coding RNAs; aging; bioinformatics.; peripheral nerves; transcriptome.