Transcriptome analysis and expression profiles of odorant binding proteins and chemosensory proteins in Orius sauteri

Comp Biochem Physiol Part D Genomics Proteomics. 2023 Dec:48:101137. doi: 10.1016/j.cbd.2023.101137. Epub 2023 Sep 4.

Abstract

The flower bug Orius sauteri (Heteroptera: Anthocoridae), is a polyphagous predator and a natural enemy widely used in biological pest control to micro-pests including aphids, spider mites, thrips and so on. In the present study, the transcriptome analysis of adult heads in O. sauteri were performed and identified a total of 38 chemosensory genes including 24 odorant binding proteins (OBPs) and 14 chemosensory proteins (CSPs). Subsequently, we conducted quantitative real-time PCR to detect the tissue expression level of 18 OBPs and 8 CSPs. The results showed that almost all OsauOBPs and OsauCSPs have a high expression level in the adult heads of both sexes. In addition, 5 OsauOBPs (OBP1, OBP2, OBP3, OBP4 and OBP14) have a significantly higher expressed in male heads than female, indicating that these chemosensory proteins might be involved in the male-specific behaviors such as pheromone reception and mate-seeking. This study will provide helpful reference for subsequent understanding of chemoreception mechanism in O. sauteri.

Keywords: Chemosensory genes; Expression pattern; Orius sauteri; Transcriptome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphids* / genetics
  • Arthropod Antennae / metabolism
  • Female
  • Gene Expression Profiling
  • Heteroptera* / genetics
  • Heteroptera* / metabolism
  • Insect Proteins / genetics
  • Insect Proteins / metabolism
  • Male
  • Odorants
  • Pheromones
  • Phylogeny
  • Receptors, Odorant* / genetics
  • Receptors, Odorant* / metabolism
  • Transcriptome

Substances

  • Pheromones
  • Receptors, Odorant
  • Insect Proteins