A novel technique for studying the effects of technologically processed antibodies by evaluating the rate of oxidation of ascorbic acid during the reduction of the green-blue ABTS + radical

Abstract

Since the pharmaceutical market is developing, there is a need for novel techniques for determining the physical-chemical properties of drug solutions. Drugs based on technologically processed antibodies (TPA) are an example of compounds that require a methodology for studying their effects. It has been shown that the process of external impacts during the manufacture of TPA-based drugs can induce breaking of intermolecular and intramolecular bonds in the solvent molecules, providing the emergence of new bonds with the molecules of the substance used for the manufacture of an active pharmaceutical ingredient. This article focuses on the technique applied for assessing the mentioned effect of TPA and consists in spectrophotometric observation of the oxidation process of ascorbic acid (AA) in the solution. The amount of oxidized AA was detected using ABTS·⁺(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical-cation, which, when interacting with AA, is reduced and changes the color from green-blue to colorless. This technique showed the reproducibility of statistically significant differences in the amount of oxidized AA in the presence of TPA compared to controls and can be used to detect the changes in the properties of solutions exposed to the effect of the TPA samples.

Keywords: ABTS·+ radical; Development of the technique; Quality control; Solution properties; Spectroscopy; Technologically processed antibodies.