Dense and Sparse Labelling of Mitral Cells by Oral and Intraperitoneal Routes of Tamoxifen Administration

Xiaochen Fu; Yu-Pei Huang; Sander Lindeman; Adam Mago; Izumi Fukunaga

doi:10.1007/978-1-0716-3425-7_14

Dense and Sparse Labelling of Mitral Cells by Oral and Intraperitoneal Routes of Tamoxifen Administration

Methods Mol Biol. 2023:2710:185-193. doi: 10.1007/978-1-0716-3425-7_14.

Authors

Xiaochen Fu¹, Yu-Pei Huang¹, Sander Lindeman¹, Adam Mago¹, Izumi Fukunaga²

Affiliations

¹ Sensory and Behavioural Neuroscience Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan.
² Sensory and Behavioural Neuroscience Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan. izumi.fukunaga@oist.jp.

PMID: 37688733
DOI: 10.1007/978-1-0716-3425-7_14

Abstract

Cell type-specific labelling and manipulation using Cre-driver lines have become integral to analyses of neuronal circuits in the brain. To study how mitral cells of the olfactory bulb process olfactory information and how they contribute to behavior, an inducible Cre-driver line, Lbhd2-CreER^T2, can be used. In this chapter, we describe two methods for administering tamoxifen. The first method achieves a dense recombination pattern using tamoxifen-containing food, while the second method involving an intraperitoneal injection is suited for sparse labelling.

Keywords: Cell types; Cre-ERT2; Mitral cells; Olfactory bulb; Tamoxifen.

MeSH terms

Brain*
Food*
Injections, Intraperitoneal
Olfactory Bulb
Tamoxifen / pharmacology

Substances

Tamoxifen