A study of the histopathology of collagen fleece (TachoSil) patching of tunica albuginea in the rat penis and a literature review of penile graft materials in experimental animals

Raouf M Seyam; Hammam M Mandourah; Mohamed S Kattan; Turki Al-Hussain; Waleed M Altaweel; Said A Kattan

doi:10.21037/tau-23-229

A study of the histopathology of collagen fleece (TachoSil) patching of tunica albuginea in the rat penis and a literature review of penile graft materials in experimental animals

Transl Androl Urol. 2023 Aug 31;12(8):1238-1249. doi: 10.21037/tau-23-229. Epub 2023 Aug 15.

Authors

Raouf M Seyam¹, Hammam M Mandourah¹, Mohamed S Kattan¹, Turki Al-Hussain², Waleed M Altaweel¹, Said A Kattan¹

Affiliations

¹ Department of Urology, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
² Department of Pathology, King Faisal Specialist Hospital and Reserch Center, Riyadh, Saudi Arabia.

Abstract

Background: A penile tunica defect may arise during surgery in patients with Peyronie's disease. Collagen fleece (TachoSil) has recently gained popularity in penile surgery to cover the tunica albuginea (TA) defect associated with clinical success. However, it is not known what the histological outcomes of these grafts are in the penis. We aimed to study the histopathology of the TachoSil graft in an experimental animal model for the regeneration of TA, inflammation, fibrosis, and the underlying cavernous tissue.

Methods: Six adult male Sprague Dawley rats were used. The penis was degloved through a circumferential subcoronal incision. A longitudinal 1 mm × 10 mm defect was created at the base of the lateral aspect of the penis. A TachoSil patch (Takeda, Japan) was applied to the defect. The penile skin covering was then restored. At 2 months, the rat penis was excised and examined with hematoxylin, eosin, and trichrome stains. We conducted a literature review of penile grafts in animals for comparison.

Results: Rats weighed 369.2 gm (standard deviation: 31.5). At 2 months, all rats showed normal-looking penis with complete healing, no scaring, tethering, or gross inflammatory features. Histopathology of the patch site showed fibrosis, chronic inflammation, and foreign body giant cell reaction. There was no generation of a new TA, or new vascularity. No inflammatory or pathological reaction affected the underlying corpus cavernous tissue. One rat died on the 6^th postoperative day. Postmortem showed massive multiorgan hemorrhage consistent with disseminated intravascular coagulopathy (DIC). Unlike some other reported grafts, there is no TA regeneration.

Conclusions: TachoSil patching of penile TA defect forms a distinctive barrier against inflammation, protecting the underlying corpus cavernosum. However, no regeneration of the tunica defect is observed at 2 months. DIC is a potential complication of systemic absorption of TachoSil.

Keywords: Collagen fleece; experimental animal study; fibrin sealant patch; histopathology; penile tunica albuginea graft (penile TA graft).