LncRNA CASC15 activated by TCF12 promote colorectal cancer progression via EMT

Shuai Yan; Ming Yuan; Xichen Yang; Xianting Huang; Dan Wu; Nanyao Wang; Xia Sun; Ya Qin; Yanhua Fei; Zhenguo Zhao; Qiong Wang

doi:10.2174/1386207326666230901120611

LncRNA CASC15 activated by TCF12 promote colorectal cancer progression via EMT

Comb Chem High Throughput Screen. 2023 Sep 1. doi: 10.2174/1386207326666230901120611. Online ahead of print.

Authors

Shuai Yan¹, Ming Yuan¹, Xichen Yang², Xianting Huang¹, Dan Wu¹, Nanyao Wang¹, Xia Sun¹, Ya Qin¹, Yanhua Fei¹, Zhenguo Zhao³, Qiong Wang¹

Affiliations

¹ Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University, No. 163 Shoushan Rd, Jiangyin, Jiangsu Province, 214400, China.
² Department of Pediatrics, Jiangyin People's Hospital Affiliated to Nantong University, No. 163 Shoushan Rd, Jiangyin, Jiangsu Province, 214400, China.
³ Department of General Surgery, Jiangyin People's Hospital Affiliated to Nantong University, No. 163 Shoushan Rd, Jiangyin, Jiangsu Province, 214400, China.

PMID: 37680149
DOI: 10.2174/1386207326666230901120611

Abstract

Background: Colorectal cancer (CRC) is one of the most common gastrointestinal malignancies. LncRNA CASC15 has also been found to play a vital role in malignant tumors.

Objective: Our objective is to explore the role of CASC15 in colorectal cancer and its regulation of EMT and to clarify the reasons for its up-regulated expression in CRC.

Methods: Quantitative real-time PCR was performed to evaluate the expression of CASC15 in CRC. The biology function of CASC15 on CRC was assessed by in vitro experiments, including CCK8, colony formation, transwell assays and flow cytometry. Luciferase reporter assays were used to confirm the regulation between TCF12 and CASC15. Quantitative real-time PCR and western blot analysis were used to evaluate the biomarkers associated with epithelial-mesenchymal transition (EMT).

Results: We found that CASC15 was remarkably upregulated in CRC and positively correlated with poorer relapse-free survival. CASC15 knockdown significantly suppressed the proliferation and migration of CRC. Furthermore, CASC15 downregulation mediated apoptosis of CRC. Mechanistically, TCF12 activates CASC15 transcription to mediate its up-regulation, which activates EMT and promotes CRC progression.

Conclusion: Our study identified TCF12/CASC15/EMT as a new regulatory signal axis of CRC. CASC15 may be a new molecular marker and target for CRC.

Keywords: CASC15; CRC; EMT; Migration; MigrationCRC; Proliferation; TCF12.