Antibody Selection in Solution Using Magnetic Beads

Philip Alexander Heine; Maximilian Ruschig; Nora Langreder; Esther Veronika Wenzel; Maren Schubert; Federico Bertoglio; Michael Hust

doi:10.1007/978-1-0716-3381-6_13

Antibody Selection in Solution Using Magnetic Beads

Methods Mol Biol. 2023:2702:261-274. doi: 10.1007/978-1-0716-3381-6_13.

Authors

Philip Alexander Heine¹, Maximilian Ruschig¹, Nora Langreder¹, Esther Veronika Wenzel², Maren Schubert¹, Federico Bertoglio^{1

3}, Michael Hust⁴

Affiliations

¹ Institut für Biochemie, Biotechnologie und Bioinformatik, Departments Biotechnology and Medical Biotechnology, Technische Universität Braunschweig, Braunschweig, Germany.
² Abcalis GmbH, Braunschweig, Germany.
³ Choose Life Biotech SA, Bellinzona, Switzerland.
⁴ Institut für Biochemie, Biotechnologie und Bioinformatik, Departments Biotechnology and Medical Biotechnology, Technische Universität Braunschweig, Braunschweig, Germany. m.hust@tu-braunschweig.de.

PMID: 37679624
DOI: 10.1007/978-1-0716-3381-6_13

Abstract

Antibody phage display is a valuable in vitro technology to generate recombinant, sequence-defined antibodies for research, diagnostics, and therapy. Up to now (autumn 2022), 14 FDA/EMA-approved therapeutic antibodies were developed using phage display, including the world best-selling antibody adalimumab. Additionally, recombinant, sequence-defined antibodies have significant advantages over their polyclonal counterparts.For a successful in vitro antibody generation by phage display, a suitable panning strategy is highly important. We present in this book chapter the panning in solution and its advantages over panning with immobilized antigens and give detailed protocols for the panning and screening procedure.

Keywords: Antibody phage display; Antibody selection; Biotinylated antigens; Panning; Panning in solution; Streptavidin.

MeSH terms

Antibodies*
Cell Surface Display Techniques*
Magnetic Phenomena
Seasons
Technology

Substances

Antibodies