Human alveolar bone-derived mesenchymal stem cell cultivation on a 3D-printed PDLLA scaffold for bone formation

Xu Liu; Shouyin Lv; Wenjiao Kan; Boxi Fan; Bo Shao

doi:10.1016/j.bjoms.2023.07.016

Human alveolar bone-derived mesenchymal stem cell cultivation on a 3D-printed PDLLA scaffold for bone formation

Br J Oral Maxillofac Surg. 2023 Oct;61(8):527-533. doi: 10.1016/j.bjoms.2023.07.016. Epub 2023 Aug 10.

Authors

Xu Liu¹, Shouyin Lv², Wenjiao Kan², Boxi Fan², Bo Shao³

Affiliations

¹ Department of Stomatology, Baoding First Central Hospital, 320 Great Wall North Street, Baoding 071000, Hebei, China.
² Department of Stomatology, Inner Mongolia Autonomous Region People's Hospital, 20 Zhaowuda Road, Huhhot 010017, Inner Mongolia, China.
³ Department of Stomatology, Inner Mongolia Autonomous Region People's Hospital, 20 Zhaowuda Road, Huhhot 010017, Inner Mongolia, China. Electronic address: huhhot2@163.com.

PMID: 37679196
DOI: 10.1016/j.bjoms.2023.07.016

Abstract

This study aimed to assess effects of 3-dimensionally (3D) printed poly-d,l-lactin (PDLLA) on human alveolar bone-derived mesenchymal stem cell (h-ABMSC) osteogenic proliferation and differentiation. Human ABMSCs were cultured and identified using flow cytometry and morphological analysis. Control and PDLLA experimental groups were assessed using a Cell Counting Kit-8 (CCK-8) to detect cellular cytotoxicity and proliferative activity. Real-time quantitative polymerase chain reaction was used to determine expression levels of osteogenesis genes including alkaline phosphatase (ALP), Runt-related transcription factor 2 (Runx-2), osteopontin (OPN), and osteocalcin (OCN). The results showed that h-ABMSCs were successfully cultured and revealed by microscopic observation. Human ABMSCs were spindle-shaped, with clustered and fish-like primary cells. Cell surface markers were negative for CD34 and positive for CD44 and CD90. PDLLA had no cytotoxicity. Human ABMSCs proliferated normally, and osteogenic differentiation of the cells was observed on the surface of PDLLA. Cellular proliferative activity and expression levels of osteogenesis-related genes of PDLLA and control groups showed no significant difference, including ALP, Runx-2, OPN, and OCN. These results suggest that 3D-printed PDLLA has good cell compatibility and biological activity.

Keywords: Alveolar bone defect; Human alveolar bone-derived stem cells; Osteogenic potential; PDLLA; Regenerative material.