Autophagy induced by Cyprinid herpesvirus 3 (CyHV-3) facilitated intracellular viral replication and extracellular viral yields in common carp brain cells

Wei Luo; Hemei Qi; Zhihong Huang; Min Guo; Dikuang Peng; Zimin Yang; Zihan Fan; Qing Wang; Qiwei Qin; Min Yang; Xuezhu Lee

doi:10.1016/j.fsi.2023.109049

Autophagy induced by Cyprinid herpesvirus 3 (CyHV-3) facilitated intracellular viral replication and extracellular viral yields in common carp brain cells

Fish Shellfish Immunol. 2023 Oct:141:109049. doi: 10.1016/j.fsi.2023.109049. Epub 2023 Sep 9.

Authors

Wei Luo¹, Hemei Qi², Zhihong Huang¹, Min Guo¹, Dikuang Peng¹, Zimin Yang¹, Zihan Fan¹, Qing Wang³, Qiwei Qin¹, Min Yang⁴, Xuezhu Lee⁵

Affiliations

¹ Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bio Resource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China.
² Guangzhou Jinan Biomedicine Research and Development Centre, Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China.
³ Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development of Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, 510380, Guangdong, China.
⁴ Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bio Resource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China. Electronic address: yangmin2016@scau.edu.cn.
⁵ Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bio Resource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China. Electronic address: xzlee@scau.edu.cn.

PMID: 37678483
DOI: 10.1016/j.fsi.2023.109049

Abstract

Autophagy is a conservative and important process that exists in all eukaryotic cells in nature. Cyprinid herpesvirus 3 (CyHV-3), also known as KHV (Koi Herpesvirus), is a pathogen that mainly infecting common carp and koi. In the present study, we identified the CcLC3B gene, with a length of 379 bp and displaying a close evolutionary relationship with other sixteen different species, the tissue distribution and expression pattern of CcLC3 were also identified. We found that CyHV-3 infection could promote autophagy in CCB cells at the early stage but inhibit autophagy at the late stage by using confocal fluorescence microscopy, transmission electron microscopy and western blotting. And we measured the protein levels associated with the Akt/mTOR signalling pathway, intracellular replication of CyHV-3 at the mRNA and protein levels as well as viral titters. Collectively, the results taken together suggested that CyHV-3 infection could promote autophagy in CCB cells at the early stage but inhibit autophagy at the late stage via mTOR and that promoting autophagy could facilitate CyHV-3 intracellular replication and extracellular viral yields in CCB cells. These findings revealed the relationship between CyHV-3 and autophagy and provided a novel treatment strategy targeting the autophagy signalling pathway against CyHV-3 infection.

Keywords: Akt/mTOR; Autophagy; Common carp; CyHV-3.