As the population worldwide ages, there is a growing need for assistive technology and effective human-machine interfaces to address the wider range of motor disabilities that older adults may experience. Motor disabilities can make it difficult for individuals to perform basic daily tasks, such as getting dressed, preparing meals, or using a computer. The goal of this study was to investigate the effect of two weeks of training with a myoelectric computer interface (MCI) on motor functions in younger and older adults. Twenty people were recruited in the study: thirteen younger (range: 22-35 years old) and seven older (range: 61-78 years old) adults. Participants completed six training sessions of about 2 hours each, during which the activity of right and left biceps and trapezius were mapped into a control signal for the cursor of a computer. Results highlighted significant improvements in cursor control, and therefore in muscle coordination, in both groups. All participants with training became faster and more accurate, although people in different age range learned with a different dynamic. Results of the questionnaire on system usability and quality highlighted a general consensus about easiness of use and intuitiveness. These findings suggest that the proposed MCI training can be a powerful tool in the framework of assistive technologies for both younger and older adults. Further research is needed to determine the optimal duration and intensity of MCI training for different age groups and to investigate long-term effects of training on physical and cognitive function.