Frozen Bovine Semen Storage, Semen Handling, and Site of Deposition

Vet Clin North Am Food Anim Pract. 2024 Mar;40(1):157-165. doi: 10.1016/j.cvfa.2023.08.009. Epub 2023 Sep 4.

Abstract

Artificial insemination (AI) is an effective strategy to distribute semen from elite sires and improve the genetics of a herd. Commercial studs provide a fertile semen product to their customers. Consequently, the maintenance of sire fertility is dependent upon appropriate semen storage, handing, and site of semen deposition. When retrieving a straw of frozen semen, the cane (and canister containing canes) should be held below the frost-line and limited to 8 seconds in the neck of the tank. After appropriate thawing, and continuous thermoregulation of the AI gun containing the thawed semen, semen should be deposited in the uterine body.

Keywords: Bovine; Semen; Semen deposition; Semen handling.

Publication types

  • Review

MeSH terms

  • Animals
  • Cattle
  • Cryopreservation / veterinary
  • Fertility
  • Insemination, Artificial / veterinary
  • Semen
  • Semen Preservation* / veterinary