Pediatric reference intervals for endocrine markers in healthy children and adolescents on the Liaison XL (DiaSorin) immunoassay system

J J Miller; M K Bohn; V Higgins; M Nichols; Z Mohammed-Ali; T Henderson; R Selvaratnam; L Sepiashvili; K Adeli

doi:10.1016/j.clinbiochem.2023.110644

Pediatric reference intervals for endocrine markers in healthy children and adolescents on the Liaison XL (DiaSorin) immunoassay system

Clin Biochem. 2023 Oct:120:110644. doi: 10.1016/j.clinbiochem.2023.110644. Epub 2023 Sep 4.

Authors

J J Miller¹, M K Bohn², V Higgins³, M Nichols⁴, Z Mohammed-Ali⁵, T Henderson⁶, R Selvaratnam⁷, L Sepiashvili², K Adeli⁸

Affiliations

¹ Department of Laboratory Medicine & Pathobiology, University of Toronto, Canada.
² Department of Laboratory Medicine & Pathobiology, University of Toronto, Canada; CALIPER Program, The Hospital for Sick Children, Toronto, ON, Canada.
³ DynaLIFE Medical Labs, Edmonton, AB, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
⁴ Department of Pathology and Laboratory Medicine, Schulich Medicine and Dentistry, Western University, London, ON, Canada.
⁵ Scarborough Health Network, Scarborough, ON, Canada.
⁶ CALIPER Program, The Hospital for Sick Children, Toronto, ON, Canada.
⁷ Department of Laboratory Medicine & Pathobiology, University of Toronto, Canada; Laboratory Medicine Program, Division of Clinical Biochemistry, University Health Network, Toronto, ON, Canada.
⁸ Department of Laboratory Medicine & Pathobiology, University of Toronto, Canada; CALIPER Program, The Hospital for Sick Children, Toronto, ON, Canada. Electronic address: khosrow.adeli@sickkids.ca.

PMID: 37673294
DOI: 10.1016/j.clinbiochem.2023.110644

Abstract

Objectives: Prominent physiological changes occurring throughout childhood and adolescence necessitate the consideration of age and sex in biomarker interpretation. Critical gaps exist in pediatric reference intervals (RIs) for specialized endocrine markers, despite expected influence of growth and development. The current study aimed to establish and/or verify RIs for six specialized endocrine markers on a specialized immunoassay system.

Methods: Samples were collected from healthy children and adolescents (5 to <19 years) and apparently healthy outpatients (0 to <5 years) as part of the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER). Serum samples were analysed for aldosterone, renin (plasma), thyroglobulin, anti-thyroglobulin, growth hormone, and insulin-like growth factor-1 (IGF-1) on the Liaison XL (DiaSorin) immunoassay platform. RIs (2.5th and 97.5th percentiles) were established for aldosterone, renin, thyroglobulin, anti-thyroglobulin, and growth hormone. Manufacturer-recommended pediatric RIs for IGF-1 were verified.

Results: Age-specific RIs were established for aldosterone, renin, and thyroglobulin, while no age-specific differences were observed for anti-thyroglobulin or growth hormone. IGF-1 was the only endocrine marker studied that demonstrated significant sex-specific differences. Manufacturer-recommended IGF-1 RIs were verified for children aged 6 to <19 years, while those for children aged 0 to <6 years did not verify.

Conclusions: This study marks the first time that pediatric RIs for aldosterone and renin were established in the CALIPER cohort and highlights the dynamic changes that occur in water and sodium homeostasis during the first years of life. Overall, these data will assist pediatric clinical laboratories in test result interpretation and improve clinical decision-making for patients tested using Liaison immunoassays.

Keywords: CALIPER; DiaSorin; Endocrine; Pediatric; Reference intervals.

MeSH terms

Adolescent
Aldosterone
Child
Female
Growth Hormone
Humans
Immunoassay
Insulin-Like Growth Factor I*
Male
Reference Values
Renin
Thyroglobulin*

Substances

Insulin-Like Growth Factor I
Thyroglobulin
Aldosterone
Renin
Growth Hormone