Clinical characterization of EFHD2 (swiprosin-1) in Glioma-associated macrophages and its role in regulation of immunosuppression

Wen-Zhao Zhang; Lin-Lin Chen; Shuo Yang; Yu-Ting Zhong; Xin Lu; Yan Wang; Zhi-Bin Wang; Ye Tu

doi:10.1016/j.ygeno.2023.110702

Clinical characterization of EFHD2 (swiprosin-1) in Glioma-associated macrophages and its role in regulation of immunosuppression

Genomics. 2023 Sep;115(5):110702. doi: 10.1016/j.ygeno.2023.110702. Epub 2023 Sep 7.

Authors

Wen-Zhao Zhang¹, Lin-Lin Chen², Shuo Yang², Yu-Ting Zhong², Xin Lu², Yan Wang³, Zhi-Bin Wang⁴, Ye Tu⁵

Affiliations

¹ Department of Pharmacy, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Department of Critical Care Medicine, School of Anesthesiology, Naval Medical University, Shanghai, China.
² Department of Critical Care Medicine, School of Anesthesiology, Naval Medical University, Shanghai, China.
³ School of Pharmacy, Naval Medical University, Shanghai, China. Electronic address: wangyansmmu@126.com.
⁴ Department of Pharmacy, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Department of Critical Care Medicine, School of Anesthesiology, Naval Medical University, Shanghai, China. Electronic address: methyl@smmu.edu.cn.
⁵ Department of Pharmacy, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200092, China. Electronic address: dan16065@163.com.

PMID: 37673235
DOI: 10.1016/j.ygeno.2023.110702

Abstract

Glioblastoma has been extensively studied due to its high mortality and short survival. The evolution mechanism of tumor-associated macrophages (TAMs) to Glioma-associated microglia and macrophages (GAMs) in the tumor microenvironment (TME) remains to be elucidated. The tumor cell-to-cell interaction patterns have not been well defined yet. The EF-Hand Domain Family Member D2 (EFHD2) has been reported to be differentially expressed as an immunomodulatory molecule in a variety of cancers. But large-scale clinical data from multiple ethnic communities have not been used to investigate the role of EFHD2 in glioma. RNA-seq data from 313 or 657 glioma patients from the Chinese Glioma Genome Atlas (CGGA) database and 603 glioma patients from the Cancer Genome Atlas (TCGA) database were analyzed retrospectively. Cell localization was performed using single-cell sequencing data from the CGGA database and the GSE131928 dataset. Mouse glioma cell lines and primary macrophages isolated from Efhd2 knockout mice were co-cultured to validate the immunomodulatory effects of EFHD2 on macrophages and the remodeling of TME of glioblastoma. EFHD2 is enriched in high-grade gliomas, isocitrate dehydrogenase wild-type, and 1p/19q non-co-deficient gliomas. It is a potential biomarker of glioma-proneuronal subtypes and an independent prognostic factor for overall survival in patients with malignant glioblastoma. EFHD2 regulates the monocyte-macrophage system function and positively correlates with immunosuppressive checkpoints. Further experimental data demonstrates that Efhd2 influences the polarization state of GAMs and inhibits the secretion of TGF-β1. In vitro experiments have revealed that macrophages lacking Efhd2 suppress the vitality of two glioma cell lines and decelerate the growth of glioma xenografts. In conclusion, EFHD2 promises to be a key target for TME-related immunotherapy.

Keywords: EF-Hand Domain Family Member D2 (EFHD2); Glioma; Macrophage.