Recent advances in the colorimetric and fluorescence analysis of bioactive small-molecule compounds based on the enzyme-like activity of nanomaterials

Abstract

Nanomaterials with enzyme-like activity have been widely used in the construction of colorimetric and fluorescence sensors due to their advantages of cost-effectiveness, high stability, good biocompatibility, and ease of modification. Furthermore, the colorimetric and fluorescence sensors, which are effective approaches for detecting bioactive small-molecule compounds, have been extensively explored due to their simple operation and high sensitivity. Recent significant researches have focused on designing various sensors based on nanozymes with peroxidase- and oxidase-like activity for the colorimetric and fluorescence analysis of different analytes. In this review, recent developments (from 2018 to present) in the colorimetric and fluorescent analysis of bioactive small-molecule compounds based on the enzyme-like activity of nanomaterials were summarized. In addition, the challenges and design strategies in developing colorimetric and fluorescent assays with high performance and specific sensing were discussed.

Keywords: Bioactive small-molecule compounds; Colorimetric sensor; Fluorescence sensor; Nanozymes.