Rat epiblast-derived stem cells recapitulate the attributes of pre-gastrulation epiblast

V Pragathi Masamsetti; Patrick P L Tam

doi:10.1016/j.crmeth.2023.100575

Rat epiblast-derived stem cells recapitulate the attributes of pre-gastrulation epiblast

Cell Rep Methods. 2023 Aug 28;3(8):100575. doi: 10.1016/j.crmeth.2023.100575.

Authors

V Pragathi Masamsetti^{1

2}, Patrick P L Tam^{1

2}

Affiliations

¹ Embryology Research Unit, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia.
² The University of Sydney, Faculty of Medicine and Health, School of Medical Sciences, NSW 2006, Australia.

Abstract

Iwatsuki and colleagues have generated self-renewing pluripotent stem cells from the pre-gastrulation epiblast of the rat embryo and from other cellular sources: rat embryonic stem cells (rESCs) and epiblast-like cells derived from the rESCs. These rat epiblast-derived stem cells (rEpiSCs) display germ-line competence that is characteristic of mouse formative stem cells and early signature of specification of germ layer lineages typical of primed state mouse epiblast stem cells.

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MeSH terms

Animals
Embryo, Mammalian
Embryonic Stem Cells
Gastrulation*
Germ Layers
Mice
Pluripotent Stem Cells*
Rats