Native promoter-mediated transcriptional regulation of crucial oleosin protein OLE1 from Prunus sibirica for seed development and high oil accumulation

Jinhe Hu; Feng Chen; Junxin Zang; Zhi Li; Jing Wang; Zirui Wang; Lingling Shi; Yu Xiu; Shanzhi Lin

doi:10.1016/j.ijbiomac.2023.126650

Native promoter-mediated transcriptional regulation of crucial oleosin protein OLE1 from Prunus sibirica for seed development and high oil accumulation

Int J Biol Macromol. 2023 Dec 31;253(Pt 1):126650. doi: 10.1016/j.ijbiomac.2023.126650. Epub 2023 Sep 2.

Authors

Jinhe Hu¹, Feng Chen¹, Junxin Zang¹, Zhi Li², Jing Wang¹, Zirui Wang¹, Lingling Shi¹, Yu Xiu³, Shanzhi Lin⁴

Affiliations

¹ College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, Beijing 100083, China.
² College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, Beijing 100083, China. Electronic address: lizhi0806@bjfu.edu.cn.
³ College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, Beijing 100083, China. Electronic address: yuxiu@bjfu.edu.cn.
⁴ College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, Beijing 100083, China. Electronic address: szlin@bjfu.edu.cn.

PMID: 37666400
DOI: 10.1016/j.ijbiomac.2023.126650

Abstract

Oleosin (OLE) is vital to stabilize lipid droplet for seed triacylglycerol (TAG) storage. This work aimed to determine key OLE and to unravel mechanism that governed seed oil accumulation of Prunus sibirica for developing biodiesel. An integrated assay of global identification of LD-related protein and the cross-accessions/developing stages comparisons associated with oil accumulative amount and OLE transcript level was performed on seeds of 12 plus trees of P. sibirica to identify OLE1 (15.5 kDa) as key oleosin protein crucial for high seed oil accumulation. The OLE1 gene and its promoter were cloned from P. sibirica seeds, and overexpression of PsOLE1 in Arabidopsis was conducted under the controls of native promoter and constitutive CaMV35S promoter, respectively. PsOLE1 promoter had seed-specific cis-elements and showed seed specificity, by which PsOLE1 was specifically expressed in seeds. Ectopic overexpression of PsOLE1, especially driven by its promoter, could facilitate seed development and oil accumulation with an increase in unsaturated FAs, and upregulate transcript of TAG assembly enzymes, but suppress transcript of LD/TAG-hydrolyzed lipases and transporters, revealing a role of native promoter-mediated transcription of PsOLE1 in seed development and oil accumulation. PsOLE1 and its promoter have considerable potential for engineering oil accumulation in oilseed plants.

Keywords: Biodiesel; Lipid droplet protein; Oil accumulation; Oleosin; Prunus sibirica seed; Seed specific promoter.

MeSH terms

Arabidopsis* / genetics
Arabidopsis* / metabolism
Gene Expression Regulation
Gene Expression Regulation, Plant
Plant Oils / metabolism
Promoter Regions, Genetic / genetics
Prunus*
Seeds

Substances

Plant Oils