NADH Photosynthesis System with Affordable Electron Supply and Inhibited NADH Oxidation

Yu Chen; Jiafu Shi; Yizhou Wu; Zheyuan Guo; Shihao Li; Wenping Li; Zhenhua Wu; Hongjian Wang; Haifei Jiang; Zhongyi Jiang

doi:10.1002/anie.202310238

NADH Photosynthesis System with Affordable Electron Supply and Inhibited NADH Oxidation

Angew Chem Int Ed Engl. 2023 Oct 16;62(42):e202310238. doi: 10.1002/anie.202310238. Epub 2023 Sep 13.

Authors

Yu Chen¹, Jiafu Shi^{1

2

3}, Yizhou Wu^{4

5}, Zheyuan Guo⁴, Shihao Li⁴, Wenping Li⁴, Zhenhua Wu¹, Hongjian Wang⁴, Haifei Jiang⁴, Zhongyi Jiang^{4

3

6}

Affiliations

¹ School of Environmental Science and Engineering, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, P. R. China.
² State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 10090, China.
³ Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, China.
⁴ School of Chemical Engineering and Technology, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, P. R. China.
⁵ Center of Artificial Photosynthesis for Solar Fuels, School of Science, Westlake University, Hangzhou, 310024, China.
⁶ Joint School of National University of Singapore and Tianjin University, International Campus of Tianjin University Binhai New City, Fuzhou, 350207, China.

PMID: 37665568
DOI: 10.1002/anie.202310238

Abstract

Photosynthesis offers a green approach for the recycling of nicotinamide cofactors primarily NADH in bio-redox reactions. Herein, we report an NADH photosynthesis system where the oxidation of biomass derivatives is designed as an electron supply module (ESM) to afford electrons and superoxide dismutase/catalase (SOD/CAT) cascade catalysis is designed as a reactive oxygen species (ROS) elimination module (REM) to inhibit NADH degradation. Glucose as the electron donor guarantees the reaction sustainability accompanied with oxidative products of gluconic acid and formic acid. Meanwhile, enzyme cascades of SOD/CAT greatly eliminate ROS, leading to a ≈2.00-fold elevation of NADH yield (61.1 % vs. 30.7 %). The initial reaction rate and turnover frequency (TOF) increased by 2.50 times and 2.54 times, respectively, compared with those systems without REM. Our study establishes a novel and efficient platform for NADH photosynthesis coupled to biomass-to-chemical conversion.

Keywords: Biomass Oxidation; NADH Oxidation; NADH Photosynthesis; ROS Elimination.

Abstract

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