The effect of GelDex-S58 hydrogel on anti-conjunctival scarring after glaucoma filtration surgery

Yi Lin; Wangdu Luo; Bingcai Jiang; Qianyi Lin; Min Tang; Xiangji Li; Lin Xie

doi:10.1016/j.isci.2023.107633

The effect of GelDex-S58 hydrogel on anti-conjunctival scarring after glaucoma filtration surgery

iScience. 2023 Aug 16;26(9):107633. doi: 10.1016/j.isci.2023.107633. eCollection 2023 Sep 15.

Authors

Yi Lin¹, Wangdu Luo¹, Bingcai Jiang¹, Qianyi Lin¹, Min Tang¹, Xiangji Li¹, Lin Xie¹

Affiliation

¹ Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Abstract

Excessive scarring is the main cause of surgical failure in glaucoma filtration surgery. S58 has been shown to have an excellent antifibrotic effect but its duration of action is not sufficient to achieve the desired antiscarring effect. In this study, a light-cured bioadhesive hydrogel composed of GelMA and oxidized dextran (ODex), namely, GelDex, was used to load S58 (GelDex-S58). The microscopic morphology of GelDex-S58 appeared to be a porous structure with good slow-release properties and suitable degradation time. Cell Counting Kit-8, cell scratch and transwell assays showed that GelDex-S58 significantly reduced TGF-β-induced fibroblast proliferation, increased migration and invasion ability. In in vivo studies, GelDex-S58 treatment prolonged follicular retention, reduced mean intraocular pressure, and significantly reduced collagen deposition and α-SMA expression levels in the conjunctival tissue compared to treatment with S58 alone. In conclusion, GelDex-S58 could reduce scar formation after glaucoma filtration surgery.

Keywords: Cancer; Surgery; Surgical procedure.