Meiotic recombination is confirmed to be unusually high in the fission yeast Schizosaccharomyces pombe

Qichao Lian; Laetitia Maestroni; Maxime Gaudin; Bertrand Llorente; Raphael Mercier

doi:10.1016/j.isci.2023.107614

Meiotic recombination is confirmed to be unusually high in the fission yeast Schizosaccharomyces pombe

iScience. 2023 Aug 11;26(9):107614. doi: 10.1016/j.isci.2023.107614. eCollection 2023 Sep 15.

Authors

Qichao Lian¹, Laetitia Maestroni², Maxime Gaudin², Bertrand Llorente², Raphael Mercier¹

Affiliations

¹ Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Cologne, Germany.
² CNRS UMR7258, INSERM U1068, Aix Marseille Université UM105, Institut Paoli-Calmettes, CRCM, Marseille, France.

Abstract

In most eukaryotes, meiotic crossovers (COs) are limited to 1-3 per chromosome, and are prevented from occurring close to one another by CO interference. The fission yeast Schizosaccharomyces pombe, an exception to these general rules, was reported to have the highest CO number per chromosome and no or weak interference. However, global CO frequency was indirectly estimated, calling for confirmation. Here, we used an innovative strategy to determine COs genome-wide in S. pombe. We confirmed weak CO interference, acting at physical distances compatible with the patterning of recombination precursors. We revealed a slight co-variation in CO number between chromosomes, suggesting that a limiting pro-CO factor varies between meiocytes. CO number per chromosome varies proportionally with chromosome size, with the three chromosomes having, on average, 15.9, 12.5, and 7.0 COs, respectively. This reinforces S. pombe's status as the eukaryote with the highest CO number per chromosome described to date.

Keywords: Cell biology.