Regulation of HDAC11 gene expression in early myogenic differentiation

Qiao Li; Yan Z Mach; Munerah Hamed; Saadia Khilji; Jihong Chen

doi:10.7717/peerj.15961

Regulation of HDAC11 gene expression in early myogenic differentiation

PeerJ. 2023 Aug 30:11:e15961. doi: 10.7717/peerj.15961. eCollection 2023.

Authors

Qiao Li^{1

2}, Yan Z Mach¹, Munerah Hamed¹, Saadia Khilji¹, Jihong Chen²

Affiliations

¹ Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
² Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.

Abstract

Histone acetylation and deacetylation affect the patterns of gene expression in cellular differentiation, playing pivotal roles in tissue development and maintenance. For example, the intrinsic histone acetyltransferase activity of transcriptional coactivator p300 is especially required for the expression of myogenic regulatory factors including Myf5 and MyoD, and consequently for skeletal myogenesis. On the other hand, histone deacetylases (HDACs) remove the acetyl group from histones, which is critical for gene repression in stem cell fate transition. Through integrative omic analyses, we found that while some HDACs were differentially expressed at the early stage of skeletal myoblast differentiation, Hdac11 gene expression was significantly enhanced by nuclear receptor signaling. In addition, p300 and MyoD control Hdac11 expression in milieu of normal and signal-enhanced myoblast differentiation. Thus, HDAC11 may be essential to differential gene expression at the onset of myoblast differentiation.

Keywords: Chromatin state; Gene regulation; Histone acetylation; Histone deacetylase; Myogenic differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylation
Cell Differentiation / genetics
Gene Expression
Histone Deacetylases* / genetics
Histones*

Substances

Histone Deacetylases
Histones

Grants and funding

This research was supported by an Operating Grant from the Natural Sciences and Engineering Research Council of Canada to QL (NSERC #250174). Munerah Hamed is a recipient of a scholarship from the Umm Al-Qura University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.