BNC-rSS, a bivalent subunit nanovaccine affords the cross-protection against Streptococcus agalactiae and Streptococcus iniae infection in tilapia

Jia Liu; Gaoyang Liu; Ye Cao; Hui Du; Tianqiang Liu; Mingzhu Liu; Pengfei Li; Yang He; Gaoxue Wang; Qing Yu; Erlong Wang

doi:10.1016/j.ijbiomac.2023.126670

BNC-rSS, a bivalent subunit nanovaccine affords the cross-protection against Streptococcus agalactiae and Streptococcus iniae infection in tilapia

Int J Biol Macromol. 2023 Dec 31;253(Pt 1):126670. doi: 10.1016/j.ijbiomac.2023.126670. Epub 2023 Sep 1.

Authors

Jia Liu¹, Gaoyang Liu¹, Ye Cao¹, Hui Du¹, Tianqiang Liu², Mingzhu Liu³, Pengfei Li³, Yang He⁴, Gaoxue Wang⁵, Qing Yu⁶, Erlong Wang⁷

Affiliations

¹ Northwest A&F University Shenzhen Research Institute, Shenzhen, Guangdong 518000, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
² Northwest A&F University Shenzhen Research Institute, Shenzhen, Guangdong 518000, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Shaanxi 712100, China.
³ Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, China.
⁴ Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, Sichuan 641000, China.
⁵ Northwest A&F University Shenzhen Research Institute, Shenzhen, Guangdong 518000, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: wanggx138@126.com.
⁶ Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, China. Electronic address: yuqing@gxas.cn.
⁷ Northwest A&F University Shenzhen Research Institute, Shenzhen, Guangdong 518000, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: welsicau@126.com.

PMID: 37660857
DOI: 10.1016/j.ijbiomac.2023.126670

Abstract

Streptococcal disease has severely restricted the development of global tilapia industry, which is mainly caused by Streptococcus agalactiae (S. agalactiae) and Streptococcus iniae (S. iniae). Vaccination has been proved to be a potential strategy to control it. In this study, a multi-epitope subunit vaccine Sip-Srr (SS) was prepared based on the B-cell antigenic epitopes prediction and multiple sequence alignment analysis of Sip and Srr sequences. Furthermore, the BNC-rSS nanocarrier vaccine system was constructed by connecting the rSS protein with modified bacterial nanocellulose (BNCs) and characterized by Fourier Transform Infrared Spectroscopy and Scanning Electron Microscope, the immersion immune effect against S. agalactiae and S. iniae infection was evaluated. The results showed that compared with the control group, BNC-rSS significantly enhanced serum antibody production, related enzyme activities and immune-related genes expression. It was noteworthy that BNC-rSS vaccine improved immune protection of tilapia, with survival rates of 66.67 % (S. agalactiae) and 60.00 % (S. iniae), respectively, compared with those of rSS vaccine (30 % and 33.33 %, respectively). Our study indicated that the BNC-rSS nanovaccine could elicit robust immune responses in tilapia by immersion immunization, and had the potential to offer cross-protection against S. agalactiae and S. iniae infection in tilapia.

Keywords: Bacterial nanocellulose; Bivalent nanovaccine; Immersion immunization.

MeSH terms

Animals
Bacterial Vaccines
Cichlids*
Fish Diseases*
Streptococcal Infections* / prevention & control
Streptococcal Infections* / veterinary
Streptococcus agalactiae
Streptococcus iniae
Tilapia*

Substances

Bacterial Vaccines