Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice

Jun Shi; DongXue Ma; ShanHu Gao; Fei Long; Xin Wang; XingYu Pu; Richard D Cannon; Ting-Li Han

doi:10.3389/fmicb.2023.1219763

Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice

Front Microbiol. 2023 Aug 15:14:1219763. doi: 10.3389/fmicb.2023.1219763. eCollection 2023.

Authors

Jun Shi^{1

2}, DongXue Ma^{1

2}, ShanHu Gao^{1

2}, Fei Long^{1

2}, Xin Wang^{1

2}, XingYu Pu^{1

2}, Richard D Cannon³, Ting-Li Han^{1

2

4}

Affiliations

¹ State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China.
² Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, China.
³ Department of Oral Sciences, Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand.
⁴ Department of Obstetrics and Gynaecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Abstract

Introduction: Obesity and diabetes are common chronic metabolic disorders which can cause an imbalance of the intestinal flora and gut-liver metabolism. Several studies have shown that probiotics, including Escherichia coli Nissle 1917 (EcN), promote microbial balance and metabolic health. However, there are no studies on how EcN outer membrane vesicles (EcN-OMVs) influence the intestinal microflora and affect the metabolic disorders of obesity and diabetes.

Methods: In this study, we evaluated the effects of EcN-OMVs on high-fat diet (HFD)-induced obesity and HFD + streptozotocin (STZ)-induced diabetes.

Results: EcN-OMVs could reduce body weight, decrease blood glucose, and increase plasma insulin in obese mice. Similarly, EcN-OMVs treatment could modify the ratio of Firmicutes/Bacteroidetes in the gut, elevate intestinal short-chain fatty acid (SCFA)-producing flora, and influence the SCFA content of the intestine. Furthermore, the intestinal metabolites ornithine and fumaric acid, hepatic ω-6 unsaturated fatty acids, and SCFAs were significantly increased after administering EcN-OMVs.

Discussion: Overall, this study showed that EcN-OMVs might act as post-biotic agents that could modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes.

Keywords: Escherichia coli Nissle 1917; diabetes; gut microbiota; metabolomics; obesity; outer membrane vesicles; probiotics.