Interactive effects of polyethylene microplastics and cadmium on growth of Glycine max

Abstract

The interaction of microplastics (MPs) and heavy metals (HMs) can lead to aggravation of detrimental effects in the plants, animals, and even human beings. Keeping this in view, the present study was designed to assess the combined toxic effects of polyethylene MPs (PE-MPs) and cadmium (Cd) on germination indices and seedling growth of soybean (Glycine max). Particle sizes of 13 and 6.5 μm and six treatments (control, Cd, 6.5 μm PE, 6.5 μm PE + Cd, 13 μm PE, and 13 μm PE + Cd) were set to simulate the effects of PE-MPs and Cd on the growth of soybean when used alone or in combined form. As compared to the control, 6.5 μm PE treatment showed significant effect on most of the germination indices, i.e., decrease in the germination index by 31%, 44% decrease in the vigor index, and 28% decrease in germination rate whereas mean germination time showed no significant differences. Treatment of smaller-size PE-MPs and Cd significantly inhibited both dry and fresh weights. All treatment groups resulted in significant effect on catalase, peroxidase, and superoxide dismutase activities of seedlings depicting adverse effects of interaction of PE-MPs and Cd. Our findings demonstrated the phyto-toxicity of PE-MPs and Cd in G. max, and it would lead to serious implications in human beings. Our study is important as it provides preliminary information regarding MP absorption and their accumulation in different levels of food chain. It can also form the basis for future research on single the combined effects of different types and sizes of MPs and heavy metals on the terrestrial plants.

Keywords: Cadmium; Catalase; Germination; Glycine max; Heavy metals; Interaction; Microplastics; Phyto-toxicity; Polyethylene; SOD; Seedling growth.