Higher temperature and daily fluctuations aggravate clothianidin toxicity towards Limnodrilus hoffmeisteri

Yingnan Wang; Jiatao Shen; Xin Li; Hongbin Lang; Luqing Zhang; Hua Fang; Yunlong Yu

doi:10.1016/j.scitotenv.2023.166655

Higher temperature and daily fluctuations aggravate clothianidin toxicity towards Limnodrilus hoffmeisteri

Sci Total Environ. 2023 Dec 10:903:166655. doi: 10.1016/j.scitotenv.2023.166655. Epub 2023 Aug 28.

Authors

Yingnan Wang¹, Jiatao Shen¹, Xin Li¹, Hongbin Lang¹, Luqing Zhang¹, Hua Fang¹, Yunlong Yu²

Affiliations

¹ Institute of Pesticide and Environmental Toxicology, the Key Laboratory of Molecular Biology of Crop Pathogens and Insects, College of Agricultural and Biotechnology, Zhejiang University, Hangzhou 310058, China.
² Institute of Pesticide and Environmental Toxicology, the Key Laboratory of Molecular Biology of Crop Pathogens and Insects, College of Agricultural and Biotechnology, Zhejiang University, Hangzhou 310058, China. Electronic address: ylyu@zju.edu.cn.

PMID: 37647951
DOI: 10.1016/j.scitotenv.2023.166655

Abstract

In nature, aquatic organisms may suffer from chemical pollution, together with thermal stress resulted from global warming. However, limited information is available on the combined effects of pesticide with climate change on aquatic organisms. In this study, the acute toxicity of clothianidin to Limnodrilus hoffmeisteri as well as its effect on the induction of oxidative stress under both constant temperature and daily temperature fluctuation (DTF) regimes was investigated. Results showed that clothianidin exhibited the minimal toxicity to L. hoffmeisteri at 25 °C, which was magnified by both increased or decreased temperatures and 10 °C DTF. At different temperatures (15 °C, 25 °C and 35 °C), clothianidin exposure led to the elevated reactive oxygen species (ROS) levels and activated the antioxidant enzymes to resist against the oxidative stress. However, the antioxidant response induced by clothianidin was overwhelmed at high temperature as evidenced by decreased glutathione (GSH) content. Significant elevation of catalase (CAT) and peroxidase (POD) activities but depletion of GSH was also observed in worms treated with clothianidin under DTF after 24 h. The results indicated that high temperature and DTF could aggravate the clothianidin-induced oxidative stress. Moreover, the critical thermal maximum (CT_max) of the worms decreased with the increasing clothianidin concentrations, suggesting that exposure to clothianidin could reduce the heat tolerance of L. hoffmeisteri. Our work highlights the crucial importance to integrate temperature changes into risk assessment of pesticides under global warming.

Keywords: Daily temperature fluctuation; Heat tolerance; Oxidative stress; Temperature-dependent toxicity.