The complete chloroplast genome and phylogenomic analysis of Camellia sinensis var. sinensis cultivar 'Liupao', a landrace from Guangxi, China

Mitochondrial DNA B Resour. 2023 Aug 25;8(8):921-926. doi: 10.1080/23802359.2023.2250072. eCollection 2023.

Abstract

Liupao tea is one of the well-known Chinese tea brands and a famous local specialty in Wuzhou, Guangxi, China. However, the genetic background and phylogenetic relationship of the native resource plants of Liupao tea need study, especially at the genomic level. In this study, we reported the complete chloroplast (cp) genome sequence of Camellia sinensis var. sinensis cultivar 'Liupao' (LP, Liupao tea population) and inferred its phylogenetic relationship to other tea plant variants or cultivars. The cp genome had a total length of 157,097 bp and the overall GC content was 37.3%. The cp genome contained one LSC region (86,641 bp) and one SSC region (18,276 bp), which were separated by two IR regions (26,090 bp, respectively). Moreover, the cp genomes were composed of 130 genes, including 86 protein-coding genes, 36 tRNA genes, and eight rRNA genes. The phylogenetic analysis showed that LP was closely related to C. sinensis var. pabilimba cv. 'Lingyunbaihao'. This study will provide useful information for further investigating the genetic background, evolution, and breeding of LP as well as other tea cultivars and varieties.

Keywords: Camellia sinensis; Liupao tea; chloroplast genome; phylogeny.

Grants and funding

This study was supported by Guangxi Natural Science Foundation Project [2023GXNSFAA026221], Guangxi Innovation-Driven Development Special Fund Project [AA20302018-1], Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain [22-035-26], Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization [FPRU2020-4], Guangxi Central Guiding development of local science and technology [ZY21195056].