Salylic acid minimize cadmium accumulation in rice through regulating the fixation capacity of the cell wall to cadmium

Zhi Jian Chen; Jing Huang; Su Li; Ji Feng Shao; Ren Fang Shen; Xiao Fang Zhu

doi:10.1016/j.plantsci.2023.111839

Salylic acid minimize cadmium accumulation in rice through regulating the fixation capacity of the cell wall to cadmium

Plant Sci. 2023 Nov:336:111839. doi: 10.1016/j.plantsci.2023.111839. Epub 2023 Aug 27.

Authors

Zhi Jian Chen¹, Jing Huang², Su Li², Ji Feng Shao¹, Ren Fang Shen², Xiao Fang Zhu³

Affiliations

¹ State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China; College of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou 311300, China; College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China.
² State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China; College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China; University of Chinese Academy of Sciences, Beijing 100049, China.
³ State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China; College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: xiaofangzhu@issas.ac.cn.

PMID: 37643701
DOI: 10.1016/j.plantsci.2023.111839

Abstract

Although salylic acid (SA) has been linked to how plants react to cadmium (Cd) stress, the exact mechanism is still unknown. The endogenous SA concentration in the rice (Oryza sativa L.) roots was enhanced by Cd stress in the current investigation, and exogenous SA reduced the hemicellulose content in root cell wall, which in turn inhibited its Cd binding capacity. What's more, exogenous SA also decreased the transcription level of genes such as Natural Resistance-Associated Macrophage Protein 5 (OsNRAMP5) and a major facilitator superfamily gene-OsCd1 that responsible for root Cd absorption. Finally, less Cd was accumulated in the rice as a result of the higher expression of Heavy Metal ATPase 3 (OsHMA3), Cation/Ca exchanger 2 (OsCCX2) and Pleiotropic Drug Resistance 9 (OsPDR9/OsABCG36) that were responsible for separating Cd into vacuole and getting Cd out of cells, respectively. In contrast, mutant with low SA level accumulated more Cd. Additionally, SA enhanced endogenous nitric oxide (NO) levels, and its alleviatory effects were mimicked by a NO donor, sodium nitroprusside (SNP). In conclusion, SA enhanced rice's Cd resistance through regulating the binding capacity of the cell wall to Cd, a pathway that might dependent on the NO accumulation.

Keywords: Cd stress; Cell wall; NO; Rice; SA.