Selective Microbial Conversion of DHEA into 7α-OH-DHEA

Vyacheslav V Kollerov; Andrei A Shutov; Marina V Donova

doi:10.1007/978-1-0716-3385-4_16

Selective Microbial Conversion of DHEA into 7α-OH-DHEA

Methods Mol Biol. 2023:2704:269-275. doi: 10.1007/978-1-0716-3385-4_16.

Authors

Vyacheslav V Kollerov¹, Andrei A Shutov¹, Marina V Donova¹

Affiliation

¹ Federal Research Center "Pushchino Center for Biological Research of the Russian Academy of Sciences", G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia.

PMID: 37642850
DOI: 10.1007/978-1-0716-3385-4_16

Abstract

7α-Hydroxy dehydroepiandrosterone (7α-OH-prasterone, 7α-OH-DHEA) is a key steroid intermediate in the synthesis of valuable pharmaceuticals widely used in the treatment of autoimmune illness, rheumatoid arthritis, colitis, and other severe diseases. The steroid can be produced using a filamentous fungus, which is capable of regio- and stereospecific hydroxylation of the steroid 3β-alcohol (DHEA) in the allylic position C7. Here, we describe a method for highly selective microbial production of 7α-OH-DHEA from DHEA using the zygomycete Backusella lamprospora VKM F-944. The method ensures high yield of 7α-OH-DHEA (up to 89%, mol/mol) even at high concentration of the substrate DHEA (15 g/L).

Keywords: 7α-Hydroxylation; 7α-OH-DHEA; Backusella lamprospora; Biocatalysis; DHEA; Filamentous fungi; Steroids; Transformation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arthritis, Rheumatoid*
Colitis*
Dehydroepiandrosterone
Ethanol
Humans
Hydroxylation

Substances

Ethanol
Dehydroepiandrosterone