Efficient genome editing in cotton using the virus-mediated CRISPR/Cas9 and grafting system

Wei-Feng Guo; Dan-Dan Guo; Fen Li; Shen-Zhai Shang; Ting-Wan Li; Ying-Chao Tang; Man Jiang; Fu-Chun Xu; Wei Gao

doi:10.1007/s00299-023-03061-2

Efficient genome editing in cotton using the virus-mediated CRISPR/Cas9 and grafting system

Plant Cell Rep. 2023 Nov;42(11):1833-1836. doi: 10.1007/s00299-023-03061-2. Epub 2023 Aug 29.

Authors

Wei-Feng Guo^#¹, Dan-Dan Guo^#², Fen Li², Shen-Zhai Shang², Ting-Wan Li², Ying-Chao Tang², Man Jiang², Fu-Chun Xu³, Wei Gao⁴

Affiliations

¹ Agricultural College, Tarim University, Alaer, Xinjiang, People's Republic of China.
² National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China.
³ Changzhi Medical College, Changzhi, Shanxi, People's Republic of China.
⁴ National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China. gaowei021@163.com.

^# Contributed equally.

PMID: 37642675
DOI: 10.1007/s00299-023-03061-2

Abstract

The extensive application of CRISPR in cotton was limited due to the labor-intensive transformation process. Thus, we here established a convenient method of CRISPR in cotton by CLCrV-mediated sgRNA delivery.

Grants and funding

202203021222310/Shanxi Basic Research Program