Catechol-Modified and MnO2-Nanozyme-Reinforced Hydrogel with Improved Antioxidant and Antibacterial Capacity for Periodontitis Treatment

Shanshan Hu; Liping Wang; Jiao Li; Dize Li; Huan Zeng; Tao Chen; Lingjie Li; Xuerong Xiang

doi:10.1021/acsbiomaterials.3c00454

Catechol-Modified and MnO₂-Nanozyme-Reinforced Hydrogel with Improved Antioxidant and Antibacterial Capacity for Periodontitis Treatment

ACS Biomater Sci Eng. 2023 Sep 11;9(9):5332-5346. doi: 10.1021/acsbiomaterials.3c00454. Epub 2023 Aug 29.

Authors

Shanshan Hu¹, Liping Wang¹, Jiao Li¹, Dize Li¹, Huan Zeng¹, Tao Chen¹, Lingjie Li¹, Xuerong Xiang¹

Affiliation

¹ Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing 401147, P. R. China.

PMID: 37642176
DOI: 10.1021/acsbiomaterials.3c00454

Abstract

Periodontitis is an inflammatory disease characterized by tooth loss and alveolar bone resorption. Bacteria are the original cause of periodontitis, and excess reactive oxygen species (ROS) encourage and intensify inflammation. In this study, a mussel-inspired and MnO₂ NPs-reinforced adhesive hydrogel capable of alleviating periodontitis with improved antibacterial and antioxidant abilities was developed. The hydrogel was created by combining polyvinyl alcohol (PVA), 3,4-dihydroxy-d-phenylalanine (DOPA), and MnO₂ nanoparticles (NPs) (named PDMO hydrogel). The hydrogel was demonstrated to be able to scavenge various free radicals (including total ROS─O₂^•- and OH^•) and relieve the hypoxia in an inflammatory microenvironment by scavenging excess ROS and generating O₂ due to its superoxide dismutase (SOD)/catalase (CAT)-like activity. Besides, under 808 nm near-infrared (NIR) light, the photothermal performance of the PDMO hydrogel displayed favorable antibacterial and antibiofilm effects toward Escherichia coli, Staphylococcus aureus, and Porphyromonas gingivalis (up to nearly 100% antibacterial rate). Furthermore, the PDMO hydrogel exhibited favorable therapeutic efficacy in alleviating gingivitis in Sprague-Dawley rats, even comparable to or better than the commercial PERIO. In addition, in the periodontitis models, the PDMO2 group showed the height of the residual alveolar bone and the smallest shadow area of low density among other groups, indicating the positive role of the PDMO2 hydrogel in bone regeneration. Finally, the biosafety of the PDMO hydrogel was comprehensively investigated, and the hydrogel was demonstrated to have good biocompatibility. Therefore, the developed PDMO hydrogel provided an effective solution to resolve biofilm recolonization and oxidative stress in periodontitis and could be a superior candidate for local drug delivery system in the clinical management of periodontitis with great potential for future clinical translation.

Keywords: MnO2 nanoparticle; antibacterial; antioxidant; hydrogel; mussel; periodontitis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / administration & dosage
Anti-Bacterial Agents / pharmacology
Antioxidants / administration & dosage
Antioxidants / pharmacology
Bacteria / drug effects
Biofilms / drug effects
Bone Regeneration / drug effects
Hydrogels* / administration & dosage
Hydrogels* / chemical synthesis
Hydrogels* / pharmacology
Periodontitis* / drug therapy
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism

Substances

catechol
manganese dioxide
Hydrogels
Anti-Bacterial Agents
Antioxidants
Reactive Oxygen Species