Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue

Dalila Juliana Silva Ribeiro; Seniz Yüksel; Andreas Dolf; Dagmar Wachten

doi:10.1007/978-1-0716-3437-0_10

Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue

Methods Mol Biol. 2024:2713:149-158. doi: 10.1007/978-1-0716-3437-0_10.

Authors

Dalila Juliana Silva Ribeiro¹, Seniz Yüksel¹, Andreas Dolf², Dagmar Wachten³

Affiliations

¹ Institute of Innate Immunity, Department of Biophysical Imaging, Medical Faculty, University of Bonn, Bonn, Germany.
² Flow Cytometry Core Facility, Medical Faculty, University of Bonn, Bonn, Germany.
³ Institute of Innate Immunity, Department of Biophysical Imaging, Medical Faculty, University of Bonn, Bonn, Germany. dwachten@uni-bonn.de.

PMID: 37639121
DOI: 10.1007/978-1-0716-3437-0_10

Abstract

Macrophages are one of the prominent leukocyte populations in white adipose tissue (WAT) and play an important role during WAT homeostasis and remodeling. Macrophage function in WAT is determined by ontogeny and the local tissue environment. Here, we present a protocol to analyze different macrophage populations from murine WAT using flow cytometry.

Keywords: Flow cytometry; Macrophages; White adipose tissue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue, White*
Animals
Flow Cytometry
Homeostasis
Leukocytes*
Macrophages
Mice