Identification of potential functional peptides involved in demyelinating injury in the central nervous system

Xiaohua Dong; Shuchen Sun; Jie Li; Sen Shen; Wanting Chen; Tongqi Li; Xinyuan Li

doi:10.7717/peerj.15846

Identification of potential functional peptides involved in demyelinating injury in the central nervous system

PeerJ. 2023 Aug 21:11:e15846. doi: 10.7717/peerj.15846. eCollection 2023.

Authors

Xiaohua Dong¹, Shuchen Sun¹, Jie Li¹, Sen Shen¹, Wanting Chen², Tongqi Li³, Xinyuan Li¹

Affiliations

¹ Department of Neurosurgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² Jiangnan University, Wuxi, China.
³ Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Abstract

Multiple sclerosis (MS) is a chronic inflammatory neurologic disease characterized by the demyelinating injury of the central nervous system (CNS). It was reported that the mutant peptide came from myelin proteolipid protein (PLP) and myelin basic protein (MBP) might play a critical role in immunotherapy function of MS. However, endogenous peptides in demyelinating brain tissue of MS and their role in the pathologic process of MS have not been revealed. Here, we performed peptidomic analysis of freshly isolated corpus callosum (CC) from the brains of CPZ-treated mice and normal diet controls of male C57BL/6 mice by LC-MS/MS. Identified a total of 217 peptides were expressed at different levels in MS mice model compared with controls. By performed GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis, we found that the precursor protein of these differently expressed peptides (DEPs) were associated with myelin sheath and oxidative phosphorylation. Our study is the first brain peptidomic of MS mice model, revealing the distinct features of DEPs in demyelination brain tissue. These DPEs may provide further insight into the pathogenesis and complexity of MS, which would facilitate the discovery of the potential novel and effective strategy for the treatment of MS.

Keywords: Central nervous system; Demyelinating injury; MBP; Multiple sclerosis; Peptides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Central Nervous System
Chromatography, Liquid
Disease Models, Animal
Male
Mice
Mice, Inbred C57BL
Multiple Sclerosis*
Peptides / genetics
Tandem Mass Spectrometry*

Substances

Peptides

Grants and funding

This work was supported by the Natural Science Foundation of Shanghai under Grant No. 22ZR1457100, and the Famous doctor’s workshop in Changning District of Shanghai (MYGZS007). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.