HPLC-DAD optimization of quantification of vescalagin, gallic and ellagic acid in chestnut tannins

Charline Richard-Dazeur; Philippe Jacolot; Céline Niquet-Léridon; Luc Goethals; Nicolas Barbezier; Pauline M Anton

doi:10.1016/j.heliyon.2023.e18993

HPLC-DAD optimization of quantification of vescalagin, gallic and ellagic acid in chestnut tannins

Heliyon. 2023 Aug 7;9(8):e18993. doi: 10.1016/j.heliyon.2023.e18993. eCollection 2023 Aug.

Authors

Charline Richard-Dazeur¹, Philippe Jacolot¹, Céline Niquet-Léridon¹, Luc Goethals², Nicolas Barbezier¹, Pauline M Anton¹

Affiliations

¹ Institut Polytechnique UniLaSalle, Université d'Artois, ULR 7519, Beauvais, France.
² Sanluc International nv, Langerbruggekaai 1, 9000, Gent, Belgium.

Abstract

The quantification of hydrolysable polyphenols such as gallic, ellagic acid and vescalagin by HPLC-DAD is classically run after methanol extraction as a reference solvent. Water extraction is usually discarded because of a lower obtention of total polyphenol content compared to methanol extraction. In our study, methanol was compared to water extraction in both the total polyphenol content method and the HPLC-DAD analysis. Total polyphenol content in water extraction was lower than in methanol extraction, but water extraction gave better results on HPLC-DAD. In conclusion, total polyphenol content cannot be used as reference to choose the solvent of extraction to quantify some polyphenols by HPLC-DAD because of the specific properties of each polyphenol. Indeed, recovery results obtained on hydrolysable polyphenols with water extraction were better and with a lower variability than following methanol extraction.

Keywords: Chestnut tannins; Ellagic acid; Gallic acid; HPLC-DAD; Polyphenol; Vescalagin.