Mead acid production by disruption of Δ12-desaturase gene in Mortierella alpina 1S-4

Hiroshi Kikukawa; Akinori Ando; Asuka Hannya; Mohd Fazli Farida Asras; Tomoyo Okuda; Takaiku Sakamoto; Kiyotaka Y Hara; Eiji Sakuradani; Jun Ogawa

doi:10.1016/j.jbiosc.2023.08.001

Mead acid production by disruption of Δ12-desaturase gene in Mortierella alpina 1S-4

J Biosci Bioeng. 2023 Nov;136(5):353-357. doi: 10.1016/j.jbiosc.2023.08.001. Epub 2023 Aug 25.

Authors

Hiroshi Kikukawa¹, Akinori Ando², Asuka Hannya³, Mohd Fazli Farida Asras³, Tomoyo Okuda³, Takaiku Sakamoto⁴, Kiyotaka Y Hara⁵, Eiji Sakuradani⁴, Jun Ogawa⁶

Affiliations

¹ Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan; Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
² Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan; Research Unit for Physiological Chemistry, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
³ Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
⁴ Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8513, Japan.
⁵ Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
⁶ Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan; Research Unit for Physiological Chemistry, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: ogawa.jun.8a@kyoto-u.ac.jp.

PMID: 37635046
DOI: 10.1016/j.jbiosc.2023.08.001

Abstract

Mead acid (MA; 20:3ω9) is one of the ω9 series of polyunsaturated fatty acids (PUFAs). MA is used to inhibit the inflammation of joints and is applied to the medicinal or health food field. We aimed to construct MA-producing strains with disruption of the Δ12-desaturase gene (Δ12ds) via an efficient gene-targeting system using the lig4-disrupted strain of Mortierella alpina 1S-4 as the host. The transformants showed a unique fatty acid composition that only comprised ω9-PUFAs and saturated fatty acids, while ω6-and ω3-PUFAs were not detected, and the total composition of ω9-PUFAs, including oleic acid (18:1ω9), 18:2ω9, 20:1ω9, 20:2ω9, and MA, was up to 68.4% of the total fatty acids. The MA production in the Δ12ds-disruptant reached 0.10 g/L (8.5%), which exceeded 0.050 g/L (4.6%) in the conventional Δ12ds-defective mutant JT-180.

Keywords: Gene disruption; Mead acid; Mortierella alpina 1S-4; Polyunsaturated fatty acid; Δ12-desaturase.