Hyperpolarized [2-13C]pyruvate MR molecular imaging with whole brain coverage

Brian T Chung; Yaewon Kim; Jeremy W Gordon; Hsin-Yu Chen; Adam W Autry; Philip M Lee; Jasmine Y Hu; Chou T Tan; Chris Suszczynski; Susan M Chang; Javier E Villanueva-Meyer; Robert A Bok; Peder E Z Larson; Duan Xu; Yan Li; Daniel B Vigneron

doi:10.1016/j.neuroimage.2023.120350

Hyperpolarized [2-¹³C]pyruvate MR molecular imaging with whole brain coverage

Neuroimage. 2023 Oct 15:280:120350. doi: 10.1016/j.neuroimage.2023.120350. Epub 2023 Aug 25.

Authors

Affiliations

¹ Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; UCSF - UC Berkeley Graduate Program in Bioengineering, University of California, USA.
² Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA. Electronic address: yaewon.kim@ucsf.edu.
³ Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA.
⁴ ISOTEC Stable Isotope Division, MilliporeSigma, Merck KGaA, Miamisburg, OH 45342, USA.
⁵ Department of Neurological Surgery, University of California, San Francisco, CA 94158, USA.
⁶ Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; UCSF - UC Berkeley Graduate Program in Bioengineering, University of California, USA; Department of Neurological Surgery, University of California, San Francisco, CA 94158, USA.

PMID: 37634883
PMCID: PMC10530049 (available on 2024-10-15)
DOI: 10.1016/j.neuroimage.2023.120350

Abstract

Hyperpolarized (HP) ¹³C Magnetic Resonance Imaging (MRI) was applied for the first time to image and quantify the uptake and metabolism of [2-¹³C]pyruvate in the human brain to provide new metabolic information on cerebral energy metabolism. HP [2-¹³C]pyruvate was injected intravenously and imaged in 5 healthy human volunteer exams with whole brain coverage in a 1-minute acquisition using a specialized spectral-spatial multi-slice echoplanar imaging (EPI) pulse sequence to acquire ¹³C-labeled volumetric and dynamic images of [2-¹³C]pyruvate and downstream metabolites [5-¹³C]glutamate and [2-¹³C]lactate. Metabolic ratios and apparent conversion rates of pyruvate-to-lactate (k_PL) and pyruvate-to-glutamate (k_PG) were quantified to investigate simultaneously glycolytic and oxidative metabolism in a single injection.

Keywords: Brain Metabolism; Hyperpolarized carbon-13; Molecular imaging.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Brain / diagnostic imaging
Glutamic Acid
Humans
Lactic Acid
Magnetic Resonance Imaging*
Molecular Imaging
Pyruvic Acid*

Substances

Pyruvic Acid
Glutamic Acid
Lactic Acid

Abstract

Publication types

MeSH terms

Substances

Grants and funding