Lnc-Malat1 promotes slow myofiber-type transformation through sponging miR-129-5p in C2C12 myotubes

Yongqi Yue; Yuhe Wang; Chenglong Wen; Yingying Meng; Ying Peng; Xiao Li

doi:10.1016/j.yexcr.2023.113761

Lnc-Malat1 promotes slow myofiber-type transformation through sponging miR-129-5p in C2C12 myotubes

Exp Cell Res. 2023 Oct 1;431(1):113761. doi: 10.1016/j.yexcr.2023.113761. Epub 2023 Aug 25.

Authors

Yongqi Yue¹, Yuhe Wang², Chenglong Wen³, Yingying Meng⁴, Ying Peng⁵, Xiao Li⁶

Affiliations

¹ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Shaanxi, China. Electronic address: yyq08053611@163.com.
² Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Shaanxi, China. Electronic address: ajcuiw@163.com.
³ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Shaanxi, China. Electronic address: wenchenglong2022@163.com.
⁴ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Shaanxi, China. Electronic address: myy1202x@163.com.
⁵ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Shaanxi, China. Electronic address: ypengxa@hotmail.com.
⁶ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Shaanxi, China. Electronic address: nice.lixiao@gmail.com.

PMID: 37634561
DOI: 10.1016/j.yexcr.2023.113761

Abstract

Long non-coding metastasis-associated lung adenocarcinoma transcript (lnc-Malat1) emerges as a novel regulator in skeletal muscle development, while its function and the related mechanism is not fully revealed yet. In this study, knockdown of lnc-Malat1 by siRNA significantly inhibited the expression of myoblast marker genes (MyHC, MyoD, and MyoG) and slow muscle fiber marker genes (MyHC I), together with repressed expression of mitochondria-related genes COX5A, ACADM, CPTA1, FABP3, and NDUFA1. Overexpression of lnc-Malat1 exerted an opposite effect, promoting myoblast differentiation and slow muscle fiber formation. Dual luciferase reporter assay revealed a direct interaction between lnc-Malat1 and miR-129-5p, and overexpression of lnc-Malat1 significantly inhibited miR-129-5p expression, thereby elevating the expression of Mef2a, miR-129-5p target protein. In addition, enforced expression of lnc-Malat1 restored the inhibitory effect of miR-129-5p on myoblast differentiation and MyHC I expression. Taken together, our results suggest that lnc-Malat1 promotes myoblast differentiation, and maintains the slow muscle fiber phenotype via adsorbing miR-129-5p.

Keywords: Lnc-Malat1; Myoblast differentiation; Myofiber type; miR-129-5p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Assay
Cell Differentiation / genetics
DNA, Mitochondrial
MicroRNAs* / genetics
Muscle Fibers, Skeletal*

Substances

DNA, Mitochondrial
MicroRNAs