Recent advances in Zn2+ imaging: From organelles to in vivo applications

Hongbao Fang; Yaheng Li; Xiuzhi Yang; Yuncong Chen; Zijian Guo; Weijiang He

doi:10.1016/j.cbpa.2023.102378

Recent advances in Zn²⁺ imaging: From organelles to in vivo applications

Curr Opin Chem Biol. 2023 Oct:76:102378. doi: 10.1016/j.cbpa.2023.102378. Epub 2023 Aug 24.

Authors

Hongbao Fang¹, Yaheng Li², Xiuzhi Yang², Yuncong Chen³, Zijian Guo⁴, Weijiang He⁵

Affiliations

¹ State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China. Electronic address: fangh2@nju.edu.cn.
² State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
³ State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China; Nanchuang (Jiangsu) Institute of Chemistry and Health, Nanjing 210000, China. Electronic address: chency@nju.edu.cn.
⁴ State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China; Nanchuang (Jiangsu) Institute of Chemistry and Health, Nanjing 210000, China.
⁵ State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China; Nanchuang (Jiangsu) Institute of Chemistry and Health, Nanjing 210000, China. Electronic address: heweij69@nju.edu.cn.

PMID: 37633062
DOI: 10.1016/j.cbpa.2023.102378

Abstract

Zn²⁺ is involved in various physiological and pathological processes in living systems. Monitoring the dynamic spatiotemporal changes of Zn²⁺ levels in organelles, cells, and in vivo is of great importance for the investigation of the physiological and pathological functions of Zn²⁺. However, this task is quite challenging since Zn²⁺ in living systems is present at low concentrations and undergoes rapid dynamic changes. In this review, we summarize the design and application of fluorescent probes for Zn²⁺ imaging in organelles, cells, and live organisms reported over the past two years. We aim to provide inspiration for the design of novel Zn²⁺ probes for multi-level monitoring and deepen the understanding of Zn²⁺ biology.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Fluorescent Dyes*
Organelles*
Zinc

Substances

Fluorescent Dyes
Zinc