Platinum Palladium Bimetallic Nanozymes Stabilized with Vancomycin for the Sensitive Colorimetric Determination of L-cysteine

Han Zhao; Kai Liu; Lijie Zhou; Tingting Zhang; Zengsheng Han; Longgang Wang; Xianbing Ji; Yanshuai Cui; Jie Hu; Guanglong Ma

doi:10.3390/biom13081254

Platinum Palladium Bimetallic Nanozymes Stabilized with Vancomycin for the Sensitive Colorimetric Determination of L-cysteine

Biomolecules. 2023 Aug 16;13(8):1254. doi: 10.3390/biom13081254.

Authors

Han Zhao^{1

2}, Kai Liu¹, Lijie Zhou¹, Tingting Zhang¹, Zengsheng Han¹, Longgang Wang^{1

2}, Xianbing Ji³, Yanshuai Cui³, Jie Hu¹, Guanglong Ma⁴

Affiliations

¹ Hebei Key Laboratory of Nano-Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
² State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China.
³ Department of Environmental Engineering, Hebei University of Environmental Engineering, Qinhuangdao 066102, China.
⁴ Centre for Cancer Immunology, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, UK.

Abstract

Many diseases in the human body are related to the level of L-cysteine. Therefore, it is crucial to establish an efficient, simple and sensitive platform for L-cysteine detection. In this work, we synthesized platinum palladium bimetallic nanoparticles (Van-Pt_m/Pd_n NPs) using vancomycin hydrochloride (Van) as a stabilizer, which exhibited high oxidase-like catalytic activity. In addition, the catalytic kinetics of the Van-Pt₁/Pd₁ NPs followed the typical Michaelis-Menten equation, exhibiting a strong affinity for 3,3',5,5'-tetramethylbenzidine substrates. More importantly, we developed a simple and effective strategy for the sensitive colorimetric detection of L-cysteine using biocompatible Van-Pt₁/Pd₁ NPs. The detection limit was low, at 0.07 μM, which was lower than the values for many previously reported enzyme-like detection systems. The colorimetric method of the L-cysteine assay had good selectivity. The established method for the detection of L-cysteine showed promise for biomedical analysis.

Keywords: L-cysteine; bimetallic; colorimetric; detection; nanozymes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry*
Cysteine
Humans
Palladium
Platinum
Vancomycin*

Substances

Vancomycin
Cysteine
Palladium
Platinum

Grants and funding

This research was funded by the Science and Technology Project of Hebei Education Department (QN2022124), Natural Science Foundation of Hebei Province (B2017203229, H2022203004), Key Program of Hebei University of Environmental Engineering (2020ZRZD02), and Yanshan University’s Specialty (Medical Engineering Interdisciplinarity) Cultivation Project (UH202209).