Genomic insights into a multidrug-resistant Acinetobacter bereziniae strain co-carrying blaOXA-301 and blaNDM-1 from China

Abstract

Objectives: Acinetobacter bereziniae has been found to cause health care-associated infections, especially in immunocompromised patients. The emergence of two carbapenemase-producing A. bereziniae strains complicates clinical management. Here, we present the genome sequence of a clinical A. bereziniae strain from China co-carrying bla_OXA-301 and bla_NDM-1.

Methods: The genomic DNA of BZAB1 was subjected to whole-genome sequencing using the Illumina NovaSeq 6000 system and assembled using SPAdes 3.13.0. Using the resfinder database of ABRicate V1.01, antimicrobial resistance genes were identified. The Snippy application was used to carry out the phylogenetic analysis.

Results: The genome sequence of A. bereziniae BZAB1 consists of 122 contigs consisting of 4 596 983 bp. A total of nine antimicrobial resistance genes were predicted in BZAB1, including two carbapenemase genes: bla_OXA-301 and bla_NDM-1. Sixty-nine A. bereziniae strains can be retrieved from the National Centre for Biotechnology Information database, 29 of which possess the bla_OXA-301 gene and five of which contain the bla_NDM-1 gene. Only three strains carry both bla_NDM-1 and bla_OXA-301. It is worth noting that all three strains carrying both bla_NDM-1 and bla_OXA-301 are from China, two of which are clonally related to BZAB1.

Conclusion: We report the genome sequence of a multidrug-resistant A. bereziniae strain co-carrying bla_OXA-301 and bla_NDM-1. A. bereziniae strains carrying various beta-lactam resistance genes have been identified sporadically over the world. Our findings could help us aid in understanding the genomic insights of this pathogen. Their future prevalence must be given more consideration.

Keywords: Acinetobacter bereziniae; Carbapenemase; NDM-1; OXA-301; Whole-genome sequencing.