Genome analysis of Tepidibacter sp. SWIR-1, an anaerobic endospore-forming bacterium isolated from a deep-sea hydrothermal vent

Xue-Gong Li; Jie Dai; Wei-Jia Zhang; Ai-Jun Jiang; Deng-Hui Li; Long-Fei Wu

doi:10.1016/j.margen.2023.101049

Genome analysis of Tepidibacter sp. SWIR-1, an anaerobic endospore-forming bacterium isolated from a deep-sea hydrothermal vent

Mar Genomics. 2023 Oct:71:101049. doi: 10.1016/j.margen.2023.101049. Epub 2023 Jun 21.

Authors

Xue-Gong Li¹, Jie Dai², Wei-Jia Zhang³, Ai-Jun Jiang⁴, Deng-Hui Li⁴, Long-Fei Wu⁵

Affiliations

¹ Laboratory of Deep-sea Microbial Cell Biology, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China; International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms, CNRS-Marseille/CAS-Sanya, China; CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China; Institution of Deep-sea Life Sciences, IDSSE-BGI, Hainan Deep-sea Technology Laboratory, China. Electronic address: xuegongli@idsse.ac.cn.
² Aix Marseille Univ, CNRS, LCB, IMM, IM2B, Marseille, France.
³ Laboratory of Deep-sea Microbial Cell Biology, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China; International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms, CNRS-Marseille/CAS-Sanya, China; CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China; Institution of Deep-sea Life Sciences, IDSSE-BGI, Hainan Deep-sea Technology Laboratory, China.
⁴ BGI-Qingdao, BGI-Shenzhen, Qingdao, Shandong, China.
⁵ International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms, CNRS-Marseille/CAS-Sanya, China; Aix Marseille Univ, CNRS, LCB, IMM, IM2B, Marseille, France. Electronic address: wu@imm.cnrs.fr.

PMID: 37620056
DOI: 10.1016/j.margen.2023.101049

Abstract

Tepidibacter sp. SWIR-1, a putative new species isolated from deep-sea hydrothermal vent field on the Southwest Indian Ridge (SWIR), is an anaerobic, mesophilic and endospore-forming bacterium belonging to the family Peptostreptococcaceae. In this study, we present the complete genome sequence of strain SWIR-1, consists of a single circular chromosome comprising 4,122,966 nucleotides with 29.25% G + C content and a circular plasmid comprising 38,843 nucleotides with 29.46% G + C content. In total, 3861 protein coding genes, 104 tRNA genes and 46 rRNA genes were obtained. SWIR-1 genome contains numerous genes related to sporulation and germination. Compared with the other three Tepidibacter species, SWIR-1 contained more spore germination receptor proteins. In addition, SWIR-1 contained more genes involved in chemotaxis and two-component systems than other Tepidibacter species. These results indicated that SWIR-1 has developed versatile adaptability to the Southwest Indian Ridge hydrothermal vent environment. The genome of strain SWIR-1 will be helpful for further understanding adaptive strategies used by bacteria dwelling in the deep-sea hydrothermal vent environments of different oceans.

Keywords: Complete genome; Deep-sea hydrothermal vent; Environmental adaptation; Tepidibacter.

MeSH terms

Anaerobiosis
Clostridiaceae
Hydrothermal Vents*
Nucleotides

Substances

Nucleotides