Development of a label-free impedimetric aptasensor for the detection of Acinetobacter baumannii bacteria

Rokhsareh Abedi; Jahan Bakhsh Raoof; Mojtaba Mohseni; Ayemeh Bagheri Hashkavayi

doi:10.1016/j.ab.2023.115288

Development of a label-free impedimetric aptasensor for the detection of Acinetobacter baumannii bacteria

Anal Biochem. 2023 Oct 15:679:115288. doi: 10.1016/j.ab.2023.115288. Epub 2023 Aug 22.

Authors

Rokhsareh Abedi¹, Jahan Bakhsh Raoof², Mojtaba Mohseni³, Ayemeh Bagheri Hashkavayi⁴

Affiliations

¹ Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
² Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran. Electronic address: j.raoof@umz.ac.ir.
³ Department of Microbiology, Faculty of Science, University of Mazandaran, Iran.
⁴ Department of Electrical and Computer Engineering, North Carolina State University, Raleigh, NC, 27606, United States.

PMID: 37619902
DOI: 10.1016/j.ab.2023.115288

Abstract

Acinetobacter baumannii (A. baumannii) is responsible for various nosocomial infections, which is known as a clinically crucial opportunistic pathogen. Therefore, rapid detection of this pathogen is critical to prevent the spread of infection and appropriate treatment. Biological detection probes, such as aptamers and synthetic receptors can be used as diagnostic layers to detect bacteria. In this work, an electrochemical aptasensor was developed for the ultrasensitive detection of A. baumannii by electrochemical impedance spectroscopy (EIS). The aptamer was immobilized on the surface of a CSPE modified with the nanocomposite Fe₃O₄@SiO₂@Glyoxal (Gly) for selective and label-free detection of A. baumannii. The charge transfers resistance (R_ct) between redox couple [Fe(CN)₆^3-/4-] and the surface of aptasensor in the Nyquist plot of EIS study was used as electroanalytical signal for detection and determination of A. baumannii. The obtained results showed that the constructed aptasensor could specifically detect A. baumannii in the concentration range from 1.0 × 10³-1.0 × 10⁸ Colony-forming unit (CFU)/mL and with a detection limit of 150 CFU/mL (S/N = 3). In addition to its sensitivity, the biosensor exhibits high selectivity over some other pathogens. Therefore, a simple, inexpensive, rapid, label-free, selective, and sensitive electrochemical aptasensor was developed to detect A. baumannii.

Keywords: Acinetobacter baumannii; Electrochemical aptasensor; Electrochemical impedance spectroscopy; Label-free.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii*
Bacteria
Dielectric Spectroscopy
Glyoxal
Oligonucleotides
Silicon Dioxide

Substances

Silicon Dioxide
Glyoxal
Oligonucleotides