QTL and PACE analyses identify candidate genes for anthracnose resistance in tomato

Carlos Lopez-Ortiz; Umesh K Reddy; Chong Zhang; Purushothaman Natarajan; Padma Nimmakayala; Vagner Augusto Benedito; Matthew Fabian; John Stommel

doi:10.3389/fpls.2023.1200999

QTL and PACE analyses identify candidate genes for anthracnose resistance in tomato

Front Plant Sci. 2023 Aug 4:14:1200999. doi: 10.3389/fpls.2023.1200999. eCollection 2023.

Authors

Carlos Lopez-Ortiz¹, Umesh K Reddy¹, Chong Zhang², Purushothaman Natarajan¹, Padma Nimmakayala¹, Vagner Augusto Benedito³, Matthew Fabian², John Stommel²

Affiliations

¹ Department of Biology, Gus R. Douglass Institute, West Virginia State University, Institute, WV, United States.
² The Genetic Improvement for Fruits & Vegetables Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, United States.
³ Division of Plant & Soil Sciences, West Virginia University, Morgantown, WV, United States.

Abstract

Anthracnose, caused by the fungal pathogen Colletotrichum spp., is one of the most significant tomato diseases in the United States and worldwide. No commercial cultivars with anthracnose resistance are available, limiting resistant breeding. Cultivars with genetic resistance would significantly reduce crop losses, reduce the use of fungicides, and lessen the risks associated with chemical application. A recombinant inbred line (RIL) mapping population (N=243) has been made from a cross between the susceptible US28 cultivar and the resistant but semiwild and small-fruited 95L368 to identify quantitative trait loci (QTLs) associated with anthracnose resistance. The RIL population was phenotyped for resistance by inoculating ripe field-harvested tomato fruits with Colletotrichum coccodes for two seasons. In this study, we identified twenty QTLs underlying resistance, with a range of phenotypic variance of 4.5 to 17.2% using a skeletal linkage map and a GWAS. In addition, a QTLseq analysis was performed using deep sequencing of extreme bulks that validated QTL positions identified using traditional mapping and resolved candidate genes underlying various QTLs. We further validated AP2-like ethylene-responsive transcription factor, N-alpha-acetyltransferase (NatA), cytochrome P450, amidase family protein, tetratricopeptide repeat, bHLH transcription factor, and disease resistance protein RGA2-like using PCR allelic competitive extension (PACE) genotyping. PACE assays developed in this study will enable high-throughput screening for use in anthracnose resistance breeding in tomato.

Keywords: PACE marker; QTL mapping; QTLseq; anthracnose; tomato.

Grants and funding

This research was supported by the US Department of Agriculture, Agricultural Research Service (NACA Agreement No. 58-8042-6-111 and No. 2022-38821-37344).