Transcriptome analysis revealed differentially expressed genes in rice functionally associated with brown planthopper defense in near isogenic lines pyramiding BPH14 and BPH15

Liang Hu; Dabing Yang; Hongbo Wang; Xueshu Du; Yanming Zhang; Liping Niu; Bingliang Wan; Mingyuan Xia; Huaxiong Qi; Tongmin Mou; Aiqing You; Jinbo Li

doi:10.3389/fpls.2023.1250590

Transcriptome analysis revealed differentially expressed genes in rice functionally associated with brown planthopper defense in near isogenic lines pyramiding BPH14 and BPH15

Front Plant Sci. 2023 Aug 8:14:1250590. doi: 10.3389/fpls.2023.1250590. eCollection 2023.

Authors

Liang Hu¹, Dabing Yang^{1

2}, Hongbo Wang^{1

2}, Xueshu Du¹, Yanming Zhang³, Liping Niu³, Bingliang Wan¹, Mingyuan Xia¹, Huaxiong Qi¹, Tongmin Mou², Aiqing You^{1

4}, Jinbo Li^{1

4}

Affiliations

¹ Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan, China.
² National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
³ State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
⁴ Hubei Hongshan Laboratory, Wuhan, China.

Abstract

Although rice has many pests, brown planthopper (BPH) in particular is known to cause substantial damage. The pyramiding application of BPH-resistance genes BPH14 and BPH15 has proven effective in enhancing rice defense against BPH. However, the molecular mechanisms underlying BPH14/BPH15-conferred resistance remain unexplained. In this investigation, we analyzed the transcriptomes of near isogenic lines (NILs) containing either BPH14 (B14), BPH15 (B15), or BPH14/BPH15 (B1415), as well as their recurrent parent (RP) 'Wushansimiao'. In total, we detected 14,492 differentially expressed genes (DEGs) across 12 mRNA profiles of resistant NILs and RP at different feeding stages. In the transcriptomic analysis, 531 DEGs appeared to be common among the resistant NILs compared to RP before and after BPH feeding. These common DEGs were enriched in defense response, phosphorylation, and salt stress response. In addition, 258 DEGs shared only in resistant NILs were obtained among the different feeding stages, which were enriched in oxidative stress response, karrikin response, and chloroplast organization. Considering the expression patterns and relevant research reports associated with these DEGs, 21 were chosen as BPH resistance candidates. In rice protoplasts, the candidate DEG OsPOX8.1 was confirmed to increase reactive oxygen species (ROS) accumulation by chemiluminescence measurement. Our results provide valuable information to further explore the defense mechanism of insect-resistant gene pyramiding lines and develop robust strategies for insect control.

Keywords: BPH14/BPH15; RNA-sequencing; brown planthopper; resistance; rice.

Grants and funding

This work was supported by grants from the Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement Foundation (grant no. 2021lzjj01), Hubei Academy of Agricultural Science Foundation (grant no. 2023NKYJJ02), Natural Science Foundation of Hubei Province (grant no. 2022CFB830), and the Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) (grant no. KF202209).