Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms

Zhiwen Liu; Kangli Guo; Liemei Yan; Kai Zhang; Ying Wang; Xiaokang Ding; Nana Zhao; Fu-Jian Xu

doi:10.1038/s41467-023-40830-9

Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms

Nat Commun. 2023 Aug 23;14(1):5132. doi: 10.1038/s41467-023-40830-9.

Authors

Zhiwen Liu^#^{1

2

3}, Kangli Guo^#^{1

2

3}, Liemei Yan^{1

2

3}, Kai Zhang^{1

2

3}, Ying Wang^{1

2

3}, Xiaokang Ding^{1

2

3}, Nana Zhao^{4

5

6}, Fu-Jian Xu^{7

8

9}

Affiliations

¹ State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
² Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing, 100029, China.
³ Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China.
⁴ State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China. zhaonn@mail.buct.edu.cn.
⁵ Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing, 100029, China. zhaonn@mail.buct.edu.cn.
⁶ Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China. zhaonn@mail.buct.edu.cn.
⁷ State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China. xufj@mail.buct.edu.cn.
⁸ Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing, 100029, China. xufj@mail.buct.edu.cn.
⁹ Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China. xufj@mail.buct.edu.cn.

^# Contributed equally.

Abstract

Safe and efficient antibacterial materials are urgently needed to combat drug-resistant bacteria and biofilm-associated infections. The rational design of nanoparticles for flexible elimination of biofilms remains challenging. Herein, we propose the fabrication of Janus-structured nanoparticles targeting extracellular polymeric substance to achieve dispersion or near-infrared (NIR) light-activated photothermal elimination of drug-resistant biofilms, respectively. Asymmetrical Janus-structured dextran-bismuth selenide (Dex-BSe) nanoparticles are fabricated to exploit synergistic effects of both components. Interestingly, Janus Dex-BSe nanoparticles realize enhanced dispersal of biofilms over time. Alternatively, taking advantage of the preferential accumulation of nanoparticles at infection sites, the self-propelled active motion induced by the unique Janus structure enhances photothermal killing effect. The flexible application of Janus Dex-BSe nanoparticles for biofilm removal or NIR-triggered eradication in vivo is demonstrated by Staphylococcus aureus-infected mouse excisional wound model and abscess model, respectively. The developed Janus nanoplatform holds great promise for the efficient elimination of drug-resistant biofilms in diverse antibacterial scenarios.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Biofilms
Disease Models, Animal
Extracellular Polymeric Substance Matrix
Mice
Multifunctional Nanoparticles*
Nanoparticles*
Wound Infection*

Substances

Anti-Bacterial Agents