Methods And Protocols For Live Imaging In Development

J Vis Exp. 2023 Jan 13:(191). doi: 10.3791/64642.

Abstract

Januschke, J., Loyer, N. Applications of immobilization of Drosophila tissues with fibrin clots for live imaging. Journal of Visualized Experiments. (166), 10.3791/61954 (2020). Li, T., Luo, L. An explant system for time-lapse imaging studies of olfactory circuit assembly in Drosophila. Journal of Visualized Experiments. (176), 10.3791/62983 (2021). Schramm, P., Hetsch, F., Meier, J. C., Koster, R. W. In vivo imaging of fully active brain tissue in awake zebrafish larvae and juveniles by skull and skin removal. Journal of Visualized Experiments. (168), 10.3791/62166 (2021). Ratke, J., Kramer, F., Strobl, F. Simultaneous live imaging of multiple insect embryos in sample chamber-based light sheet fluorescence microscopes. Journal of Visualized Experiments. (163), 10.3791/61713 (2020). Terzi, A., Alam, S. M. S., Suter, D. M. ROS live cell imaging during neuronal development. Journal of Visualized Experiments. (168), 10.3791/62165 (2021). Mutlu, A. S., Chen, T., Deng, D., Wang, M. C. Label-Free imaging of lipid storage dynamics in Caenorhabditis elegans using stimulated Raman scattering microscopy. Journal of Visualized Experiments. (171), 10.3791/61870 (2021). Boutillon, A., Escot, S., David, N. B. Deep and spatially controlled volume ablations using a two-photon microscope in the zebrafish gastrula. Journal of Visualized Experiments. (173), 10.3791/62815 (2021).

Publication types

  • Editorial
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Brain*
  • Caenorhabditis elegans
  • Drosophila
  • Time-Lapse Imaging
  • Zebrafish*