Pouteria caimito fruit derived nanoparticles inhibited the apple ring rot disease as well as extended the shelf-life of sliced apples

Mohammed A Alsaif; Chinnadurai Veeramani; Ahmed S El Newehy; Amal A Aloud; Khalid S Al-Numair

doi:10.1016/j.sjbs.2023.103744

Pouteria caimito fruit derived nanoparticles inhibited the apple ring rot disease as well as extended the shelf-life of sliced apples

Saudi J Biol Sci. 2023 Sep;30(9):103744. doi: 10.1016/j.sjbs.2023.103744. Epub 2023 Jul 20.

Authors

Mohammed A Alsaif¹, Chinnadurai Veeramani¹, Ahmed S El Newehy¹, Amal A Aloud², Khalid S Al-Numair¹

Affiliations

¹ Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
² Department of Food Sciences and Nutrition, College of Food and Agriculture Sciences, King Saud University, P.O.Box 2460, Riyadh 11451, Saudi Arabia.

Abstract

Background: Apple disease, exaggerated by Botryosphaeria dothidea, is a foremost intimidating problem for extending the apple fruit shelf-life and producing substantial economic losses for cultivators and distributors. Alternate sources are urgently needed to prevent or inhibit the ring rot infection of apple fruit instigated by Botryosphaeria dothidea.

Objective: In this current study, we premeditated to make novel organic nanoparticles as of Pouteria caimito fruit extract and calcium chloride (PCNP), which were used to evaluate the preventive outcome of Botryosphaeria dothidea-caused apple disease on postharvest apple fruits.

Results: Our findings corroborated that the fruit derived nanoparticle had been confirmed for quality and size by altered estimations such as fourier transform infrared (FTIR), UV-vis spectroscopic analysis, scanning electron microscope and energy dispersive X-ray (SEM and EDX) estimation, and dynamic light scattering (DLS) analysis. In addition, we have investigated the excellent inhibitory action of the pathogen infection in apples initiated by Botryosphaeria dothidea. The protective enzymes function was pointedly improved in nanoparticle-treated apple fruits once equated with those of control apple fruits. The catalase (CAT) and superoxide dismutase (SOD) activities were pointedly improved in nanoparticle-treated fruits when compared to those of control fruits. The shelf-life extension studies were conducted for 7 days with a fresh-cut apple. The total soluble solid, pH, weight loss, and sensory studies were analyzed, and they proved the extension of sliced apple shelf life up to 7 days.

Conclusions: The discoveries of this study provided a well-organized, harmless, and environment-friendly substitute to control the apple disease as well as the durability postponement of sliced apples 7 days or may longer.

Keywords: Antioxidants; Calcium chloride; Nanoparticles; Shelf-life; Sliced apples.